5(6)-SFX (Fluorescein), SE
Tocris Bioscience | Catalog # 6488
Key Product Details
Description
Wavelength
Product Description
5(6)-SFX (Fluorescein), SE is a green fluorescent dye for the labeling of primary amines. Comprising fluorescein, with a commonly used fluorophore, the hexanoic spacer that separates the fluorophore from the molecule to which it is conjugated and makes the NHS ester group more accessible to biopolymers for conjugation. Excitation maximum = 494 nm; emission maximum = 520 nm, extinction coefficient: 74,000 cm-1M-1.Please see the protocol for further information on protein/antibody labeling and conjugation.
Optical Data
| Emission Color | Green |
| λabs | 494 nm |
| λem | 518 nm |
| Extinction Coefficient (ε) | 70000 M-1cm-1 |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Application | Fluorescence Microscopy, Flow Cytometry, Immunofluorescence Assays (i.e. Western Blot, ELISA, etc) |
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Product Specifications for 5(6)-SFX (Fluorescein), SE
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMF | 58.65 | 100 | |
| DMSO | 58.65 | 100 |
Preparing Stock Solutions for 5(6)-SFX (Fluorescein), SE
The following data is based on the product molecular weight 586.55.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.70 mL | 8.52 mL | 17.05 mL |
| 5 mM | 0.34 mL | 1.70 mL | 3.41 mL |
| 10 mM | 0.17 mL | 0.85 mL | 1.70 mL |
| 50 mM | 0.03 mL | 0.17 mL | 0.34 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Ito Artificial human Met agonists based on macrocycle scaffolds. Nat.Commun. 2015 PMID: 25758345
Product Documents for 5(6)-SFX (Fluorescein), SE
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for 5(6)-SFX (Fluorescein), SE
For research use only
Customer Reviews for 5(6)-SFX (Fluorescein), SE
There are currently no reviews for this product. Be the first to review 5(6)-SFX (Fluorescein), SE and earn rewards!
Have you used 5(6)-SFX (Fluorescein), SE?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review