5(6)-SFX (Fluorescein), SE

Catalog # Availability Size / Price Qty
5(6)-SFX (Fluorescein), SE | CAS No. 114616-31-8 | Standard Fluorescein, Coumarin and Rhodamine-based
1 Image
Description: Green fluorescent dye for the labeling of primary amines

Chemical Name: 6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester

Purity: ≥90%

Product Details

Biological Activity

Green fluorescent dye for the labeling of primary amines. Comprising fluorescein, with a commonly used fluorophore, the hexanoic spacer that separates the fluorophore from the molecule to which it is conjugated and makes the NHS ester group more accessible to biopolymers for conjugation. Excitation maximum = 494 nm; emission maximum = 520 nm, extinction coefficient: 74,000 cm-1M-1.

Optical Data for 5(6)-SFX (Fluorescein), SE

Emission Color Green
λabs 494 nm
λem 518 nm
Extinction Coefficient (ε) 70000 M-1cm-1
Closest Laser line 488 nm
Reactive Group NHS ester
Reactivity Primary amines
Application Fluorescence Microscopy, Flow Cytometry, Immunofluorescence Assays (i.e. Western Blot, ELISA, etc)

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data

Soluble to 100 mM in DMSO and to 100 mM in DMF
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Artificial human Met agonists based on macrocycle scaffolds.
    Ito, Kenichir, Sakai, Katsuya, Suzuki, Yoshinor, Ozawa, Naoya, Hatta, Tomohisa, Natsume, Tohru, Matsumoto, Kunio, Suga, Hiroaki
    Nat Commun, 2015;6(0):6373.

Product Datasheets

Reconstitution Calculator
Molarity Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Molarity Calculator


*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).


No product specific FAQs exist for this product, however you may

View all Small Molecule FAQs

Reviews for 5(6)-SFX (Fluorescein), SE

There are currently no reviews for this product. Be the first to review 5(6)-SFX (Fluorescein), SE and earn rewards!

Have you used 5(6)-SFX (Fluorescein), SE?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review
Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.