5(6)-SFX (Fluorescein), SE
Chemical Name: 6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester
Biological ActivityGreen fluorescent dye for the labeling of primary amines. Comprising fluorescein, with a commonly used fluorophore, the hexanoic spacer that separates the fluorophore from the molecule to which it is conjugated and makes the NHS ester group more accessible to biopolymers for conjugation. Excitation maximum = 494 nm; emission maximum = 520 nm, extinction coefficient: 74,000 cm-1M-1.
Optical Data for 5(6)-SFX (Fluorescein), SE
|Extinction Coefficient (ε)||70000 M-1cm-1|
|Closest Laser line||488 nm|
|Reactive Group||NHS ester|
|Application||Fluorescence Microscopy, Flow Cytometry, Immunofluorescence Assays (i.e. Western Blot, ELISA, etc)|
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.Spectral Viewer
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Artificial human Met agonists based on macrocycle scaffolds.
Ito, Kenichir, Sakai, Katsuya, Suzuki, Yoshinor, Ozawa, Naoya, Hatta, Tomohisa, Natsume, Tohru, Matsumoto, Kunio, Suga, Hiroaki
Nat Commun, 2015;6(0):6373.
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