ABCG8 Antibody - BSA Free
Novus Biologicals | Catalog # NB400-110
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Scientific Data Images for ABCG8 Antibody - BSA Free
Western Blot: ABCG8 Antibody - BSA Free [NB400-110] -
Correlation of ABCG8 expression to the frequency of the polymorphism D19H in the Stuttgart cohort. The expression analysis was performed in human ileal mucosal biopsies. Values are given as means +/- SEM. Significance between the subgroups was analysed with Mann–Whitney U-test (nonparametric, two-tailed). P-values <0.05 were considered as statistically significant. (A) Quantitative analysis of mRNA expression is calculated as copy numbers. (GG) = wild type individual; (Gc) = carrier of p.D19H. Controls: (GG) = 80 and (Gc) = 15; gallstone carriers: (GG) = 20 and (Gc) = 9. (B) Representative Western blot images of ABCG8 protein and villin in ileal mucosa of control individuals and gallstone carriers, with and without p.D19H. Protein content was determined by densitometric analysis. The data were normalized to villin, an epithelial marker protein. Controls: (GG) = 59 and (Gc) = 11; gallstone carriers: (GG) = 11 and (Gc) = 9. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23406058), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: ABCG8 Antibody - BSA Free [NB400-110] -
Expression of cholesterol transporters in female gallstone carriers and healthy controls. The expression analysis was performed in human ileal mucosal biopsies. Values are calculated as means +/- SEM (standard error of the mean). Significance between the controls and gallstone carriers was analysed with Mann–Whitney U-test (nonparametric, two-tailed). Normal weight subgroup is defined as BMI ≤ 25.4, overweight subgroup as BMI > 25.4. P-values <0.05 were considered as statistically significant. (A-C) Quantification of mRNA expression is given as transcript numbers. ABCG5/ABCG8 = ATP-binding cassette transporter, NPC1L1 = Niemann-Pick C1-Like 1 protein *P = 0.0417, C = control subject, GS = gallstone carrier. Total: C = 98, GS = 30; normal weight: C = 57, GS = 10; overweight: C = 41, GS = 20. (D) Representative Western blot images of ABCG8, ABCG5 and NPC1L1 in ileal mucosa of gallstone carriers and controls. Protein content was determined by densitometric analysis. The data were normalized to villin, an epithelial marker protein. ABCG8: C = 70, GS = 20; ABCG5: C = 7, GS = 7; NPC1L1: C = 7, GS = 7. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23406058), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for ABCG8 Antibody - BSA Free
Western Blot
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Background: ABCG8
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Gene Symbol
Additional ABCG8 Products
Product Documents for ABCG8 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for ABCG8 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for ABCG8 Antibody - BSA Free
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Protocols
View specific protocols for ABCG8 Antibody - BSA Free (NB400-110):
Western Blot Protocol
1. Load ~50 micrograms of lysate (ie: liver membrane) onto an 8% SDS-PAGE, reducing.
2. Block the protein transferred membrane for 30 minutes in blocking buffer [5% NFDM, 10 mM Tris (pH 7.6) saline, 0.2% Tween-20] at room temperature.
3. Incubate the membrane in diluted primary anti-ABCG8 [cat# NB 400-110], 1:500 for 1 hour at room temperature.
4. Wash the membrane 3x 5 minutes in blocking buffer without milk.
5. Incubate the membrane in diluted secondary anti-Rabbit IgG conjugated to HRP.
6. Wash the membrane 3x 5 minutes in blocking buffer without milk.
7. Wash the membrane for 5 minutes in TBS.
8. Develop using West-Pico ECL (Pierce) reagent.
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