Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse (Negative)

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunohistochemistry (Negative)

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

A synthetic peptide from the N-terminal region of human ABCG8 protein. [UniProt# Q9H221]

Reactivity Notes

Does not appear to react with mouse.

Localization

Integral membrane protein; multi-pass membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ABCG8 Antibody - BSA Free

Western Blot: ABCG8 AntibodyBSA Free [NB400-110]

Western Blot: ABCG8 AntibodyBSA Free [NB400-110]

Western Blot: ABCG8 Antibody [NB400-110] - Analysis of ABCG8, using NB400-110. Samples: mouse liver microsomes from G5/G8 knockout mice, wildtype human G5/G8, and transgenic human G5/G8.
ABCG8 Antibody - BSA Free

Western Blot: ABCG8 Antibody - BSA Free [NB400-110] -

Correlation of ABCG8 expression to the frequency of the polymorphism D19H in the Stuttgart cohort. The expression analysis was performed in human ileal mucosal biopsies. Values are given as means +/- SEM. Significance between the subgroups was analysed with Mann–Whitney U-test (nonparametric, two-tailed). P-values <0.05 were considered as statistically significant. (A) Quantitative analysis of mRNA expression is calculated as copy numbers. (GG) = wild type individual; (Gc) = carrier of p.D19H. Controls: (GG) = 80 and (Gc) = 15; gallstone carriers: (GG) = 20 and (Gc) = 9. (B) Representative Western blot images of ABCG8 protein and villin in ileal mucosa of control individuals and gallstone carriers, with and without p.D19H. Protein content was determined by densitometric analysis. The data were normalized to villin, an epithelial marker protein. Controls: (GG) = 59 and (Gc) = 11; gallstone carriers: (GG) = 11 and (Gc) = 9. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23406058), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ABCG8 Antibody - BSA Free

Western Blot: ABCG8 Antibody - BSA Free [NB400-110] -

Expression of cholesterol transporters in female gallstone carriers and healthy controls. The expression analysis was performed in human ileal mucosal biopsies. Values are calculated as means +/- SEM (standard error of the mean). Significance between the controls and gallstone carriers was analysed with Mann–Whitney U-test (nonparametric, two-tailed). Normal weight subgroup is defined as BMI ≤ 25.4, overweight subgroup as BMI > 25.4. P-values <0.05 were considered as statistically significant. (A-C) Quantification of mRNA expression is given as transcript numbers. ABCG5/ABCG8 = ATP-binding cassette transporter, NPC1L1 = Niemann-Pick C1-Like 1 protein *P = 0.0417, C = control subject, GS = gallstone carrier. Total: C = 98, GS = 30; normal weight: C = 57, GS = 10; overweight: C = 41, GS = 20. (D) Representative Western blot images of ABCG8, ABCG5 and NPC1L1 in ileal mucosa of gallstone carriers and controls. Protein content was determined by densitometric analysis. The data were normalized to villin, an epithelial marker protein. ABCG8: C = 70, GS = 20; ABCG5: C = 7, GS = 7; NPC1L1: C = 7, GS = 7. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23406058), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for ABCG8 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
This ABCG8 antibody is useful for Western blot. The antibody does not appear to be applicable in immunohistochemistry.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ABCG8

ATP-binding cassette (ABC) transporter genes are involved in the regulation of the amount of dietary cholesterol retained in the body. ABCG8, expressed at high levels in the liver and intestine, normally cooperates with ABCG5 to limit intestinal absorption and promote biliary excretion of sterols. The mutated form of this transporter can lead to sterol accumulation and atherosclerosis or sitosterolemia, a rare autosomal recessive disorder, characterized by hyperabsorption of sterols and the inability to excrete sterols into bile.

Alternate Names

ATP-binding cassette sub-family G member 8, ATP-binding cassette, sub-family G (WHITE), member 8, ATP-binding cassette, sub-family G (WHITE), member 8 (sterolin 2), GBD4ATP-binding cassette, subfamily G, member 8, MGC142217, sterolin 2, sterolin-2, STSL

Gene Symbol

ABCG8

Additional ABCG8 Products

Product Documents for ABCG8 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ABCG8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ABCG8 Antibody - BSA Free

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Protocols

View specific protocols for ABCG8 Antibody - BSA Free (NB400-110):

ABCG8 Antibody:
Western Blot Protocol

1. Load ~50 micrograms of lysate (ie: liver membrane) onto an 8% SDS-PAGE, reducing.

2. Block the protein transferred membrane for 30 minutes in blocking buffer [5% NFDM, 10 mM Tris (pH 7.6) saline, 0.2% Tween-20] at room temperature.

3. Incubate the membrane in diluted primary anti-ABCG8 [cat# NB 400-110], 1:500 for 1 hour at room temperature.

4. Wash the membrane 3x 5 minutes in blocking buffer without milk.

5. Incubate the membrane in diluted secondary anti-Rabbit IgG conjugated to HRP.

6. Wash the membrane 3x 5 minutes in blocking buffer without milk.

7. Wash the membrane for 5 minutes in TBS.

8. Develop using West-Pico ECL (Pierce) reagent.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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