ABCG8 Antibody - BSA Free
Novus Biologicals | Catalog # NB400-110
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse (Negative)
Cited:
Human, Mouse
Applications
Validated:
Western Blot, Immunohistochemistry (Negative)
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
A synthetic peptide from the N-terminal region of human ABCG8 protein. [UniProt# Q9H221]
Reactivity Notes
Does not appear to react with mouse.
Localization
Integral membrane protein; multi-pass membrane protein
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ABCG8 Antibody - BSA Free
Western Blot: ABCG8 Antibody - BSA Free [NB400-110] -
Correlation of ABCG8 expression to the frequency of the polymorphism D19H in the Stuttgart cohort. The expression analysis was performed in human ileal mucosal biopsies. Values are given as means +/- SEM. Significance between the subgroups was analysed with Mann–Whitney U-test (nonparametric, two-tailed). P-values <0.05 were considered as statistically significant. (A) Quantitative analysis of mRNA expression is calculated as copy numbers. (GG) = wild type individual; (Gc) = carrier of p.D19H. Controls: (GG) = 80 and (Gc) = 15; gallstone carriers: (GG) = 20 and (Gc) = 9. (B) Representative Western blot images of ABCG8 protein and villin in ileal mucosa of control individuals and gallstone carriers, with and without p.D19H. Protein content was determined by densitometric analysis. The data were normalized to villin, an epithelial marker protein. Controls: (GG) = 59 and (Gc) = 11; gallstone carriers: (GG) = 11 and (Gc) = 9. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23406058), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: ABCG8 Antibody - BSA Free [NB400-110] -
Expression of cholesterol transporters in female gallstone carriers and healthy controls. The expression analysis was performed in human ileal mucosal biopsies. Values are calculated as means +/- SEM (standard error of the mean). Significance between the controls and gallstone carriers was analysed with Mann–Whitney U-test (nonparametric, two-tailed). Normal weight subgroup is defined as BMI ≤ 25.4, overweight subgroup as BMI > 25.4. P-values <0.05 were considered as statistically significant. (A-C) Quantification of mRNA expression is given as transcript numbers. ABCG5/ABCG8 = ATP-binding cassette transporter, NPC1L1 = Niemann-Pick C1-Like 1 protein *P = 0.0417, C = control subject, GS = gallstone carrier. Total: C = 98, GS = 30; normal weight: C = 57, GS = 10; overweight: C = 41, GS = 20. (D) Representative Western blot images of ABCG8, ABCG5 and NPC1L1 in ileal mucosa of gallstone carriers and controls. Protein content was determined by densitometric analysis. The data were normalized to villin, an epithelial marker protein. ABCG8: C = 70, GS = 20; ABCG5: C = 7, GS = 7; NPC1L1: C = 7, GS = 7. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23406058), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for ABCG8 Antibody - BSA Free
Application
Recommended Usage
Western Blot
1:500
Application Notes
This ABCG8 antibody is useful for Western blot. The antibody does not appear to be applicable in immunohistochemistry.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ABCG8
Alternate Names
ATP-binding cassette sub-family G member 8, ATP-binding cassette, sub-family G (WHITE), member 8, ATP-binding cassette, sub-family G (WHITE), member 8 (sterolin 2), GBD4ATP-binding cassette, subfamily G, member 8, MGC142217, sterolin 2, sterolin-2, STSL
Gene Symbol
ABCG8
Additional ABCG8 Products
Product Documents for ABCG8 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ABCG8 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for ABCG8 Antibody - BSA Free
Customer Reviews for ABCG8 Antibody - BSA Free
There are currently no reviews for this product. Be the first to review ABCG8 Antibody - BSA Free and earn rewards!
Have you used ABCG8 Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
View specific protocols for ABCG8 Antibody - BSA Free (NB400-110):
ABCG8 Antibody:
Western Blot Protocol
1. Load ~50 micrograms of lysate (ie: liver membrane) onto an 8% SDS-PAGE, reducing.
2. Block the protein transferred membrane for 30 minutes in blocking buffer [5% NFDM, 10 mM Tris (pH 7.6) saline, 0.2% Tween-20] at room temperature.
3. Incubate the membrane in diluted primary anti-ABCG8 [cat# NB 400-110], 1:500 for 1 hour at room temperature.
4. Wash the membrane 3x 5 minutes in blocking buffer without milk.
5. Incubate the membrane in diluted secondary anti-Rabbit IgG conjugated to HRP.
6. Wash the membrane 3x 5 minutes in blocking buffer without milk.
7. Wash the membrane for 5 minutes in TBS.
8. Develop using West-Pico ECL (Pierce) reagent.
Western Blot Protocol
1. Load ~50 micrograms of lysate (ie: liver membrane) onto an 8% SDS-PAGE, reducing.
2. Block the protein transferred membrane for 30 minutes in blocking buffer [5% NFDM, 10 mM Tris (pH 7.6) saline, 0.2% Tween-20] at room temperature.
3. Incubate the membrane in diluted primary anti-ABCG8 [cat# NB 400-110], 1:500 for 1 hour at room temperature.
4. Wash the membrane 3x 5 minutes in blocking buffer without milk.
5. Incubate the membrane in diluted secondary anti-Rabbit IgG conjugated to HRP.
6. Wash the membrane 3x 5 minutes in blocking buffer without milk.
7. Wash the membrane for 5 minutes in TBS.
8. Develop using West-Pico ECL (Pierce) reagent.
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...