Actin Antibody (9Y9L6)

Novus Biologicals | Catalog # NBP3-16885

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Key Product Details

Species Reactivity

Plant

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 9Y9L6
View all Actin Primary Antibodies »

Product Specifications

Immunogen

Recombinant Protein of Actin (plant specific)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Actin Antibody (9Y9L6)

Western Blot: Actin Antibody (9Y9L6) [NBP3-16885]

Western Blot: Actin Antibody (9Y9L6) [NBP3-16885]

Western Blot: Actin Antibody (9Y9L6) [NBP3-16885] - Western blot analysis of extracts of various cell lines, using Actin antibody (NBP3-16885) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western Blot: Actin Antibody (9Y9L6) [NBP3-16885]

Western Blot: Actin Antibody (9Y9L6) [NBP3-16885]

Western Blot: Actin Antibody (9Y9L6) [NBP3-16885] - Western blot analysis of lysates from Arabidopsis thaliana tissue using Actin antibody (NBP3-16885). Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Applications for Actin Antibody (9Y9L6)

Application
Recommended Usage

Western Blot

1:2000 - 1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 1% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Actin

Actin is a 43kDa filament protien that is ubiquitously expressed by all eukaryotic cells. This wide expression makes Actin an ideal "housekeeping gene", which may even account for up to 50% of total cellular protein. Actin microfilaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. alpha Actin expression is limited to various types of muscle, whereas beta and gamma are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia. Actin antibodies are most commonly used as controls for protein standardization or in cytoskeletan or cell cycle research.

Alternate Names

ACTA, actin, alpha 1, skeletal muscle, alpha skeletal muscle, alpha skeletal muscle actin, alpha-actin-1, ASMA, CFTD, CFTDM, MPFD, NEM1, NEM2, NEM3

Gene Symbol

ACTA1

Additional Actin Products

Product Documents for Actin Antibody (9Y9L6)

Certificate of Analysis

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Product Specific Notices for Actin Antibody (9Y9L6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Actin Antibody (9Y9L6)

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  • Q: I am interested in buying an Actin antibody to use in Western blot as a loading control. We would like to use it on rat heart samples. Can you make a recommendation?

    A:

    I would recommend the Actin Antibody (mAbGEa) catalog # NB100-74340. This antibody works great as a loading control in Western blot and has been validated for use with rat samples.

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