ADP-Sugar Pyrophosphatase/NUDT5 Antibody (10D2) - BSA Free
Novus Biologicals | Catalog # NBP3-26453
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10D2 expressed in HEK293
Format
BSA Free
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Product Specifications
Immunogen
A synthesized peptide derived from Human ADP-Sugar Pyrophosphatase/NUDT5 [UniProt Q9UKK9]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ADP-Sugar Pyrophosphatase/NUDT5 Antibody (10D2) - BSA Free
Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody (10D2) [NBP3-26453] -
Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody (10D2) [NBP3-26453] - Positive Western Blot detected in: Hela whole cell lysate.All lanes: ADP-Sugar Pyrophosphatase/NUDT5 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 25 kDa
Observed band size: 36 kDa
Flow Cytometry: ADP-Sugar Pyrophosphatase/NUDT5 Antibody (10D2) [NBP3-26453] -
Flow Cytometry: ADP-Sugar Pyrophosphatase/NUDT5 Antibody (10D2) [NBP3-26453] - Overlay Peak curve showing HepG2 cells stained with ADP-Sugar Pyrophosphatase/NUDT5 Antibody (10D2) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.Applications for ADP-Sugar Pyrophosphatase/NUDT5 Antibody (10D2) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Western Blot
1:500-1:2000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: ADP-Sugar Pyrophosphatase/NUDT5
Alternate Names
Nudix motif 5, YSA1, YSA1H
Gene Symbol
NUDT5
Additional ADP-Sugar Pyrophosphatase/NUDT5 Products
Product Documents for ADP-Sugar Pyrophosphatase/NUDT5 Antibody (10D2) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ADP-Sugar Pyrophosphatase/NUDT5 Antibody (10D2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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