Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) - Azide and BSA Free

Novus Biologicals | Catalog # H00000249-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4H1

Format

Azide and BSA Free
View all Alkaline Phosphatase, Tissue Non-Specific Primary Antibodies »

Product Specifications

Immunogen

ALPL (AAH21289.3, 29 a.a. ~ 138 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. WRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSR

Specificity

ALPL - alkaline phosphatase, liver/bone/kidney

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01]

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01]

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - Analysis of ALPL expression in transfected 293T cell line by ALPL monoclonal antibody (M01), clone 4H1.Lane 1: ALPL transfected lysate(57.3 KDa).Lane 2: Non-transfected lysate.
Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01]

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01]

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - ALPL monoclonal antibody (M01), clone 4H1 Western Blot analysis of ALPL expression in HeLa ( Cat # L013V1 ).
Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01]

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01]

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - Analysis of ALPL over-expressed 293 cell line, cotransfected with ALPL Validated Chimera RNAi ( Cat # H00000249-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ALPL monoclonal antibody (M01), clone 4H1 (Cat # H00000249-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
ELISA: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01]

ELISA: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01]

ELISA: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - Detection limit for recombinant GST tagged ALPL is approximately 0.1ng/ml as a capture antibody.

Applications

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Alkaline Phosphatase, Tissue Non-Specific

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Alternate Names

Alkaline phosphatase liver/bone/kidney isozyme, alkaline phosphatase, liver/bone/kidney, alkaline phosphatase, tissue-nonspecific isozyme, alkaline phosphomonoesterase, APTNAP, AP-TNAP, EC 3.1.3.1, FLJ40094, FLJ93059, glycerophosphatase, HOPS, liver/bone/kidney-type alkaline phosphatase, MGC161443, tissue-nonspecific ALP, TNAP, TNSALPMGC167935

Entrez Gene IDs

249 (Human)

Gene Symbol

ALPL

OMIM

146300 171760 241500 241510 (Human)

UniProt

AAH21289 (Human)

Additional Alkaline Phosphatase, Tissue Non-Specific Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) - Azide and BSA Free

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