Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) - BSA Free

Novus Biologicals | Catalog # NBP3-09106

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Porcine, Canine, Goat, Monkey, Sheep

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # W8B2B10

Format

BSA Free
View all Alkaline Phosphatase, Tissue Non-Specific Primary Antibodies »

Product Specifications

Immunogen

WERI-RB-1 retinoblastoma cell line

Specificity

The mouse monoclonal antibody W8B2B10 recognizes TNAP (tissue non-specific alkaline phosphatase), an ectoenzyme expressed mainly on embryonic stem cells, liver, bone, and kidney cells. This antibody is suitable for characterization of bone marrow-derived MSCs, iPSCs, and ESCs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images

Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) - BSA Free

Flow Cytometry: Mouse Monoclonal Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) [NBP3-09106] -

Flow Cytometry: Mouse Monoclonal Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) [NBP3-09106] - Flow cytometry surface staining pattern of human peripheral whole blood spiked with HeLa cells stained using anti-TNAP (W8B2B10) purified antibody (concentration in sample 0.3 ug/ml).
Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) - BSA Free

Flow Cytometry: Mouse Monoclonal Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) [NBP3-09106] -

Flow Cytometry: Mouse Monoclonal Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) [NBP3-09106] - Anti-Hu TNAP purified antibody (concentration in sample 0.3 ug/ml, GAM APC, red-filled histogram) binds specifically to TNAP on subpopulation of HeLa cells (upper panel), but not to surface of K562 cells (lower panel). Level of non-specific binding was assessed using cells unstained by primary antibody (GAM APC, black-dashed histogram).

Applications

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Alkaline Phosphatase, Tissue Non-Specific

Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

Alternate Names

Alkaline phosphatase liver/bone/kidney isozyme, alkaline phosphatase, liver/bone/kidney, alkaline phosphatase, tissue-nonspecific isozyme, alkaline phosphomonoesterase, APTNAP, AP-TNAP, EC 3.1.3.1, FLJ40094, FLJ93059, glycerophosphatase, HOPS, liver/bone/kidney-type alkaline phosphatase, MGC161443, tissue-nonspecific ALP, TNAP, TNSALPMGC167935

Gene Symbol

ALPL

Additional Alkaline Phosphatase, Tissue Non-Specific Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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