Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract

This protocol outlines a method for preparation of embedding organoids in Cultrex® Basement Membrane Extract (BME) and further analysis of the organoids by immunofluorescence. Some specific tips and considerations you can find in this protocol include:

  • Methods and tips for organoid culture, harvesting, and processing
  • Use of Cultrex BME for the conservation of organoid structures and other 3-D culture types
  • Challenges when immunostaining embedded organoids
  • Tips and considerations to improve organoid preservation and immunostaining when embedding in Cultrex BME

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