AP2A2 Antibody (6D5F5)

Novus Biologicals | Catalog # NBP3-33317

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 6D5F5
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 590-939 of human AP2A2 (NP_036437.1).

Sequence:
STDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEDTKRDRSVDVNGGPEPAPASTSAVSTPSPSADLLGLGAAPPAPAGPPPSSGGSGLLVDVFSDSASVVAPLAPGSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICSDDLQPNLNLQTKPVDPTVEGGAQVQQVVNIECVSDFTEAPVLNIQFRYGGTFQNVSVQLPITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEVTKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKEAVSQRLCELLSAQF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AP2A2 Antibody (6D5F5)

AP2A2 Antibody (6D5F5)

Western Blot: AP2A2 Antibody (6D5F5) [NBP3-33317] -

Western Blot: AP2A2 Antibody (6D5F5) [NBP3-33317] - Western blot analysis of various lysates using AP2A2 Rabbit mAb at1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.

Applications for AP2A2 Antibody (6D5F5)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:2000 - 1:20000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AP2A2

AP2A2 is a component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transportvia transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat componentsand appear to be involved in carg

Alternate Names

100 kDa coated vesicle protein C, Adapter-related protein complex 2 alpha-2 subunit, adaptin, alpha B, Adaptor protein complex AP-2 subunit alpha-2, adaptor-related protein complex 2, alpha 2 subunit, Alpha2-adaptin, Alpha-adaptin C, alpha-adaptin C; Huntingtin interacting protein J, AP-2 complex subunit alpha-2, Clathrin assembly protein complex 2 alpha-C large chain, clathrin-associated/assembly/adaptor protein, large, alpha 2, DKFZP564D1864, HIP9ADTAB, Huntingtin yeast partner J, Huntingtin-interacting protein 9, Huntingtin-interacting protein J, HYPJCLAPA2, KIAA0899HIP-9, Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit

Gene Symbol

AP2A2

Additional AP2A2 Products

Product Documents for AP2A2 Antibody (6D5F5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for AP2A2 Antibody (6D5F5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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