Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Apc1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 500 and 550 of human anaphase promoting complex subunit 1 using the numbering given in entry NP_073153.1 (GeneID 64682).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Apc1 Antibody - BSA Free

Western Blot: Apc1 Antibody [NB100-86985]

Western Blot: Apc1 Antibody [NB100-86985]

Western Blot: Apc1 Antibody [NB100-86985] - Detection of Human and Mouse APC1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-APC1 antibody NB100-86985 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. APC1 was also immunoprecipitated by another rabbit anti-APC1 antibody which recognizes an upstream epitope.

Applications for Apc1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Apc1

ANAPC1 is 1 of at least 10 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.[supplied by OMIM]

Alternate Names

anaphase promoting complex subunit 1, anaphase-promoting complex 1 (meiotic checkpoint regulator), anaphase-promoting complex subunit 1, APC1Testis-specific gene 24 protein, Cyclosome subunit 1, Mitotic checkpoint regulator, TSG24MCPR

Entrez Gene IDs

64682 (Human); 17222 (Mouse)

Gene Symbol

ANAPC1

UniProt

Q9H1A4 (Human)

Additional Apc1 Products

Product Documents for Apc1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Apc1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Apc1 Antibody - BSA Free

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