Apc4 Antibody (CIV1.1) - BSA Free

Novus Biologicals | Catalog # NBP2-50105

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # CIV1.1

Format

BSA Free
View all Apc4 Primary Antibodies »

Product Specifications

Immunogen

C-terminus of Apc4

Specificity

CIV1.1 may be used for the affinity purification of the Anaphase Promoting Complex (APC/C) by peptide elution. Suggested Dilution for western blot: 1:500. It is suggested to blot for at least 2 hours (wet transfer) to ensure that APC4 is transferred efficiently.Mass spectrometry was used to confirm that the whole APC/C is co-precipitated. To establish the APC4 specific bands, it is suggested to do an APC4 immunoprecipitation assay showing input, IP and FT and blot for at least 2 hours.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for Apc4 Antibody (CIV1.1) - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10 - 1:500

Western Blot

1:500
Application Notes
WB - It is suggested to blot for at least 2 hours (wet transfer) to ensure that APC4 is transferred efficiently. Mass spectrometry was used to confirm that the whole APC/C is co-precipitated. To establish the APC4 specific bands, it is suggested to do an Apc4 immunoprecipitation assay showing input, IP and FT and blot for at least 2 hours.Positive control(s): Purified APC/C by silver and mass spectrometry.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apc4

APC4 is a component of the anaphase-promoting complex (APC). The APC complex functions as an ubiquitin ligase that controls cell cycle progression and promotes the transition from metaphase to anaphase. The APC complex is composed of at least 11 APC components. The exact function of APC4 has not been determined.

Alternate Names

anaphase promoting complex subunit 4, anaphase-promoting complex subunit 4, APC4Cyclosome subunit 4

Gene Symbol

ANAPC4

Additional Apc4 Products

Product Documents for Apc4 Antibody (CIV1.1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Apc4 Antibody (CIV1.1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Apc4 Antibody (CIV1.1) - BSA Free

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