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Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1082 and 1132 of human HLA-B associated transcript 3 (NP_004630.3).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BAT3/BAG6 Antibody
Western Blot: BAT3/BAG6 Antibody [NBP1-18928]
Western Blot: BAT3/BAG6 Antibody [NBP1-18928] - Detection of Human BAT3 by Western Blot. Samples: Whole cell lysate (50, 5 and 5 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BAT3 antibody NBP1-18928 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunoprecipitation: BAT3/BAG6 Antibody [NBP1-18928]
Immunoprecipitation: BAT3/BAG6 Antibody [NBP1-18928] - Detection of human BAT3 by western blot of immunoprecipitates. Samples: Nuclear extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BAT3 antibody NBP1-18928 used for IP at 6 ug per reaction. BAT3 was also immunoprecipitated by rabbit anti-BAT3 antibody NBP1-18927. For blotting immunoprecipitated BAT3, NBP1-18928 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Immunoprecipitation: BAT3/BAG6 Antibody [NBP1-18928]
Immunoprecipitation: BAT3/BAG6 Antibody [NBP1-18928] - Detection of human BAT3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BAT3 antibody NBP1-18928 (lot NBP1-18928-2) used for IP at 6 ug per reaction. BAT3 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-18928-1) and rabbit anti-BAT3 antibody NBP1-18927. For blotting immunoprecipitated BAT3, NBP1-18928 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Applications for BAT3/BAG6 Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: BAT3/BAG6
Long Name
Large proline-rich protein BAT3
Alternate Names
BAG6, D6S52E, Protein G3, Scythe
Entrez Gene IDs
7917 (Human)
Gene Symbol
BAG6
UniProt
Additional BAT3/BAG6 Products
Product Documents for BAT3/BAG6 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BAT3/BAG6 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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