Amino Acid Sequence: AHAGRTGYDN REIVMKYIHY KLSQRGYEWD AGDVGAAPPG AAPAPGIFSS QPGHTPHPAA SRDPVARTSP LQTPAAPGAA AGPALSPVPP VVHLTLRQAG DDFSRRYRRD FAEMSSQLHL TPFTARGRFA TVVEELFRDG VNWGRIVAFF EFGGVMCVES VNREMSPLVD NIALWMTEYL NRHLHTWIQD NGGWDAFVEL YGPSMRPLFD

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Bcl-2 as determined by LAL method.

Predicted Molecular Mass

23.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Test in Process.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Bcl-2 Protein

SDS-PAGE: Recombinant Human Bcl-2 Protein [NBP2-34889]

SDS-Page: Human Bcl-2 Protein [NBP2-34889]

Formulation, Preparation, and Storage

NBP2-34889

Formulation	0.2 um filtered concentrated solution in 25 mM Tris-HCl, pH 8.0, 100mM NaCl, 1 mM DTT, Tween-80.
Preservative	30% glycerol
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Format	Carrier-Free
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20C. Avoid freeze-thaw cycles.

Background: Bcl-2

B-cell lymphoma 2 (Bcl-2) is the founding member of the Bcl-2 family and it is encoded by the BCL2 gene in human. Bcl-2 forms homodimer and heterodimers with other Bcl-2 family proteins, like BAX, BAK, BAD and Bcl-xL. Alternative splicing of Bcl-2 results in two isoforms with similar folds despite differences in anti-apoptotic activity. Bcl-2 suppresses apoptosis by controlling the mitochondrial membrane permeability. It inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Antibodies to Bcl-2 can be used with immunohistochemistry to identify cells containing the antigen. In some cases, the presence or absence of Bcl-2 staining in biopsies may be significant for the patient's prognosis or likelihood of cancer relapse. Mature human Bcl-2 shares 88 % - 90 % amino acid sequence identity with murine, rat, bovine, canine Bcl-2.

Long Name

B Cell Lymphoma/Leukemia 2

Alternate Names

Bcl2

Gene Symbol

BCL2

UniProt

P10415

Additional Bcl-2 Products

Product Documents for Recombinant Human Bcl-2 Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human Bcl-2 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.