BMAL1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-129

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BMAL1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein corresponding to residues 294-624 of human BMAL1. [UniProt# O00327]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit BMAL1 Antibody - BSA Free (NB100-129) is a polyclonal antibody validated for use in WB, Simple Western and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for BMAL1 Antibody - BSA Free

Western Blot: BMAL1 Antibody [NB100-129]

Western Blot: BMAL1 Antibody [NB100-129]

Western Blot: BMAL1 Antibody [NB100-129] - Detection of BMAL in 3T3/L1 lysates.
Simple Western: BMAL1 Antibody [NB100-129]

Simple Western: BMAL1 Antibody [NB100-129]

Simple Western: BMAL1 Antibody [NB100-129] - Image shows a specific band for BMAL1 in 0.5 mg/mL of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for BMAL1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Simple Western

1:100

Western Blot

1:100 - 1:200
Application Notes

This BMAL1 antibody is useful for Chromatin Immunoprecipitation and Western blot, where it recognizes a band at approx. 73 kDa representing BMAL1. Results were negative for ICC/IF in Hela and MCF7 cells.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in NIH-3T3 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100, apparent MW was 59 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BMAL1

BMAL1 (brain and muscle ARNT-like 1; ARNTL) is an essential core component in circadian clock machinery which is regulatory mechanism for circadian rhythms. Circadian clock genes include three period proteins (PER1, PER2 and PER3), two cryptochromes (CRY1 and CRY2), CLOCK, NPAS2 and BMAL proteins, wherein BMAL1 has a potential to heterodimerize with CLOCK or NPAS2 genes; and this neocomplex drives transcription from E-box elements (5'-CACGTG-3') found in circadian-responsive genes's promoters. PER/CRY proteins negatively regulate CLOCK/BMAL1 dimer-mediated transcription, thereby forming the feedback loop that regulates the timing of clock gene transcription. BMAL1 also associates with GNB2L1/RACK1 and PRKCA in a nuclear complex, whertein GNB2L1 and PRKCA are recruited to the complex in a circadian manner. BMAL1 undergoes acetylation (Lys-538), phosphorylation and sumoylation (Lys-259) upon dimerization with CLOCK and acetylation facilitates CRY1-mediated repression. CLOCK-BMAL1 double mutations within PAS domains leads to syngernistic desensitization to high levels of CRY on repression of CLOCK-BMAL1 transcriptional activity of PER1 and, disrupt circadian rhythmicity. Clock genes functions primarily as tumor suppressors and their abbarant expression is observed in malignant pleural mesothelioma, colorectal and breast cancer.

Long Name

Brain and Muscle ARNT-Like 1

Alternate Names

ARNTL, BHLHE5, BMAL1c, JAP3, MOP3, PASD3, TIC

Entrez Gene IDs

406 (Human)

Gene Symbol

ARNTL

UniProt

O00327

Additional BMAL1 Products

Product Documents for BMAL1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BMAL1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

View specific protocols for BMAL1 Antibody - BSA Free (NB100-129):

Procedure Guide for NB 100-129 BMAL1 Antibody:

(Anti-MOP3 aka Anti-BMAL1)

Western Blot Procedure

1. Proteins are separated by electrophoresis.

2. Proteins are transferred to nitrocellulose sheets.

3. Following the protein transfer, the filters are blocked for 1 hour at room temperature, gently shaking.

4. The filters are then incubated with a 1:100 dilution of (NB100-129) anti-MOP3 (aka: Banti-BMAL1) in PBS + 5% Dry Milk for 2 hours at room temperature, gently shaking. *

5. The filters are washed three times with TBST at room temperature.

6. The filters are then incubated with an Alkaline Phosphatase conjugated secondary antibody [Goat anti-Rabbit Alk Phos conjugated Ab from Jackson ImmunoResearch #111-055-003], diluted 1:5000 in PBS + 5% Dry Milk, for 1 hour at room temperature.

7. The filters are washed three times with TBST at room temperature.

8. Stained the filters with NBT/BCIP substrate at room temperature.

9. The filters are finally washed three times with TBST at room temperature.

* Overnight at 4 degrees C will increase sensitivity

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