Bovine SPAM1 Antibody

R&D Systems | Catalog # AF6436

R&D Systems
Discontinued Product
AF6436 has been discontinued. View all SPAM1 products.

Key Product Details

Species Reactivity

Bovine

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all SPAM1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant bovine SPAM1
Leu36-Thr497
Accession # AAI10184

Specificity

Detects bovine SPAM1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Bovine SPAM1 Antibody

Detection of Bovine SPAM1 antibody by Western Blot.

Detection of Bovine SPAM1 by Western Blot.

Western blot shows lysates of recombinant bovine SPAM1 (Catalog # 6436-GH). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Bovine SPAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6436) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SPAM1 at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Bovine SPAM1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant bovine SPAM1 (Catalog # 6436-GH)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SPAM1

Sperm adhesion molecule 1 (SPAM1), also known as PH-20 hyaluronidase (1), is encoded by one of the six hyaluronidase-like genes (1, 2, 3). SPAM1 is a GPI‑anchored enzyme located on the sperm surface and inner acrosomal membrane (4). SPAM1 degrades hyaluronic acid (HA), a major structural glycosaminoglycan found in extracellular matrices and basement membranes. The enzyme activity enables sperm to penetrate through the HA-rich cumulus cell layer surrounding the oocyte and therefore facilitates the fertilization process (5). However, detailed enzymatic analysis of this enzyme is hindered by the limited techniques/methods available to monitor the HA degradation products (6). A novel method for analyzing SPAM1 activity was utilized here. Because of the structural similarity between HA (repeating units of GlcA beta 1-3GlcNAc) and chondroitin sulfate (repeating units of GlcA beta 1-3GalNAc), the enzyme is also able to hydrolyze chondroitin sulfate. In this assay, radiolabeled chondroitin sulfate was digested with recombinant SPAM1. Degradation products were then separated using a polyacrylamide electrophoresis and visualized with an X-ray film (7). The bovine SPAM1 is 63% identical to human homologue in sequence.

References

  1. Lathrop, W.F. et al. (1990) J. Cell Biol. 111:2939.
  2. Lin, Y. et al. (1993) Proc. Natl. Acad. Sci. U.S.A. 90:10071
  3. Csoka, A.B. et al. (2001) Matrix Biol. 20:499.
  4. Phelps, B.M. et al (1988) Science 240:4860
  5. Gmachl, M. et al. (1993) FEBS Lett. 336:545.
  6. Hofinger, E.S et al. (2007) Glycobiology 17:963.
  7. Wu, Z.L. et al. (2010) BMC Biotechnol. 10:11. 8.
  8. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
  9. MacRae, I.J. et al. (2000) Biochemistry. 39:1613. 10.
  10. Wu, Z.L. et al. (2002) Faseb J. 16:539.

Long Name

Sperm Adhesion Molecule 1

Alternate Names

HYA1, HYAL3, HYAL5, PH20, SPAG15

Entrez Gene IDs

6677 (Human); 353352 (Bovine)

Gene Symbol

SPAM1

UniProt

AAI10184

Additional SPAM1 Products

Product Documents for Bovine SPAM1 Antibody

Certificate of Analysis

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Product Specific Notices for Bovine SPAM1 Antibody

For research use only

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