BTBD7 Antibody Blocking Peptide

Novus Biologicals | Catalog # NBP1-49652PEP

Novus Biologicals
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Key Product Details

Applications

Block/Neutralize, Antibody Competition
View all BTBD7 Proteins and Enzymes »

Product Specifications

Description

A BTBD7 antibody blocking peptide.

Purity

>85%, by HPLC

Application Notes

This peptide is useful as a blocking peptide for antibody NBP1-49652. For further blocking peptide related protocol, click here. Applications: Immmunodepletion, Competition Assay

Protein / Peptide Type

Antibody Blocking Peptide

Formulation, Preparation, and Storage

NBP1-49652PEP
Formulation Sodium Bicarbonate buffer, pH 8.00 containing up to 10% DMSO
Preservative No Preservative
Concentration 2.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: BTBD7

Cancer cells especially human hepatocellular carcinoma (HCC) differentially express many genes as determined by cDNA microarrays. One of these genes is Btbd7 whose function was not known. When transfected in NIH3T3 cells this gene exhibited enhanced cell proliferation activity by MTT assay. The enhanced tumorigencity was blocked by anti-sense RNA experiments (1). Recently it has been shown that Btbd7 regulate epithelial cell dynamics and branching morphogenesis. The organ development during embryogenesis requires extensive branching of epithelia via formation of cleft and buds. These branches are developed by local outgrowth or by formation of cleft that further divide epithelia in to buds. The bud or tubule extension is controlled and regulated by various growth factors. These branching finally led to formation of various organs. Cellular matrix protein fibronectin is required for salivary, lung and kidney branching (3). The edges of fibronectin translocates inwards as cleft accompanied by loss of E-cadherin in cells adjacent to fibronectin. The Btbd7 activity was noticed at cell cleft forming sites (2). The cleft formation involves buds delineation of buds by conversion of epithelial cell-cell adhesion to cell-matrix adhesion. Btbd7 provides a mechanistic link between the extracellular matrix and cleft propagation via its highly discrete expression that control epithelial cell motility by interaction with other cellular architectural and scaffolding proteins like Snail2 (slug), E-cadherin (4). The Btbd7 antibodies were generated using peptide corresponding to C-terminal domain of the human Btbd7 gene. The Btbd7 antibodies are affinity purified over immobilized antigen based affinity chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. Antibodies to Btbd7 (Btbd-701AP) will label ~144kDa protein in Western blot positive control for Btbd7 (PC-Btbd7).

Alternate Names

BTB (POZ) domain containing 7, DKFZp686N0544, FLJ10648, FUP1BTB/POZ domain-containing protein 7, KIAA1525, MGC48310

Gene Symbol

BTBD7

Additional BTBD7 Products

Product Documents for BTBD7 Antibody Blocking Peptide

Certificate of Analysis

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Product Specific Notices for BTBD7 Antibody Blocking Peptide

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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