CCDC8 Antibody

Novus Biologicals | Catalog # NBP1-50021

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all CCDC8 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 350 and 400 of human Coiled-Coil Domain Containing 8 using the numbering given in entry NP_114429.2 (GeneID 83987).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CCDC8 Antibody

Western Blot: CCDC8 Antibody [NBP1-50021]

Western Blot: CCDC8 Antibody [NBP1-50021]

Western Blot: CCDC8 Antibody [NBP1-50021] - Detection of Human CCDC8 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from 293T (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and K562 (K; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CCDC8 antibody used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCDC8 was also immunoprecipitated by rabbit anti-CCDC8 antibody which recognizes a downstream epitope. For blotting immunoprecipitated CCDC8, the ReliaBLOT (R) Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Applications for CCDC8 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CCDC8

Alternate Names

coiled-coil domain containing 8, coiled-coil domain-containing protein 8, DKFZp564K0322

Entrez Gene IDs

83987 (Human)

Gene Symbol

CCDC8

UniProt

Q9H0W5 (Human)

Additional CCDC8 Products

Product Documents for CCDC8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CCDC8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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