Recombinant Rat CCL11/Eotaxin Protein

Name: Recombinant Rat CCL11/Eotaxin Protein
Brand: Novus Biologicals
SKU: NBP2-35294
Price: 1250 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-35294

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

E. coli

Applications

Bioactivity, SDS-PAGE

View all CCL11/Eotaxin Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 74 amino acids corresponding to CCL11/Eotaxin Source: E. coli

Uniprot ID: P97545

Amino Acid Sequence: HPGSIPTSCC FTMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTKLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL11/Eotaxin as determined by LAL method.

Predicted Molecular Mass

8.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CCL11/Eotaxin protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 0.1-1.0 ug/ml

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat CCL11/Eotaxin Protein

SDS-PAGE: Recombinant Rat CCL11/Eotaxin Protein [NBP2-35294]

SDS-Page: Rat, CCL11/Eotaxin Protein [NBP2-35294]

Formulation, Preparation, and Storage

NBP2-35294

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CCL11/Eotaxin

CCL11 encoded by the gene CCL11is belonging to the CC chemokine family. It is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. CCL11 is a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. CCR3 has been identified to be a specific CCL11 receptor. CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates

Alternate Names

Eotaxin

Gene Symbol

CCL11

Additional CCL11/Eotaxin Products

Product Documents for Recombinant Rat CCL11/Eotaxin Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Rat CCL11/Eotaxin Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.