Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # 91
View all CD24 Primary Antibodies »

Product Specifications

Immunogen

Mouse heat stable antigen (HSA); Mw 35-45 kDa

Specificity

Mouse heat stable antigen (HSA); Mr 35-45 kDa

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Applications for CD24 Antibody (91) [Allophycocyanin]

Application
Recommended Usage

Flow Cytometry

<= 0.3 ug/10^6 cells

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS and a stabilizing agent

Preservative

Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD24

CD24, also known as heat stable antigen, is a phosphatidylinositol-linked cell surface glycoprotein that is expressed at all stages of B cell development and on most thymocytes. (1-3) The absence of expression from mature T cells is closely associated with their maturation from CD4+CD8+CD24+ thymocytes to either CD4+CD8-CD24- or CD4-CD8+CD24- T cells. CD24 is also present on mouse granulocytes, monocytes, Langerhans cells and erythrocytes. (4-6) Its ligand is P-selectin (CD62P). (7) CD24 may play a role in regulation of B cell proliferation and differentiation, and it has been proposed that heat stable antigen promotes homotypic adhesion between B lymphocytes. (1-3) The monoclonal antibody 91 can be used to inhibit B-cell differentiation. (8)

Alternate Names

CD24, CD24A

Gene Symbol

CD24

Additional CD24 Products

Product Documents for CD24 Antibody (91) [Allophycocyanin]

Certificate of Analysis

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Product Specific Notices for CD24 Antibody (91) [Allophycocyanin]

Protect conjugated forms from light.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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