Mouse CD31/PECAM-1 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # KA1509

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Supernatant, Plasma (EDTA, Heparin), Serum (100 uL)

Sensitivity

<10 pg/mL

Assay Range

156 to 10000 pg/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Description

Pecam1 (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of mouse Pecam1.

Scientific Data Images for Mouse CD31/PECAM-1 ELISA Kit (Colorimetric)

ELISA: Mouse CD31/PECAM-1 ELISA Kit (Colorimetric) [KA1509]

ELISA: Mouse CD31/PECAM-1 ELISA Kit (Colorimetric) [KA1509]

ELISA: CD31/PECAM-1 ELISA Kit [HRP] [KA1509] - This standard curve was generated for demonstration purpose only. A standard curve must be run with each assay.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD31/PECAM-1

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1

Gene Symbol

PECAM1

Additional CD31/PECAM-1 Products

Product Documents for Mouse CD31/PECAM-1 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Mouse CD31/PECAM-1 ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Mouse CD31/PECAM-1 ELISA Kit (Colorimetric)

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  • Q: I was wondering what the shelf life of KA1509 was? Also, is the format such that you have to use the entire plate at a time or does the plate come in strips allowed multiple experiments to be performed with the same kit?

    A: This kit has a shelf life of 6 months, stored at 4C. Also, the kit is supplied as a 96-well plate, and you will have to use an entire plate.

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