Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Armenian Hamster IgG1 Clone # H1.2F3
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Product Specifications

Immunogen

Mouse dendritic epidermal cell line Y245

Reactivity Notes

Mouse reactivity reported in (PMID: 29367423).

Specificity

Mouse CD69/Very Early Activation Antigen (VEA), Mr 85 kDa (unreduced)

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG1

Scientific Data Images for CD69 Antibody (H1.2F3) [Alexa Fluor® 488]

Product Image: CD69 Antibody (H1.2F3) [Alexa Fluor® 488] [NBP1-28011AF488] - Vial of Alexa Fluor 488 conjugated antibody. Alexa Fluor 488 is optimally excited at 490 nm by the Blue laser (488 nm) and has an emission maximum of 525 nm.

Applications for CD69 Antibody (H1.2F3) [Alexa Fluor® 488]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-28011AF488 in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD69

CD69, also known as very early activation (VEA) antigen, is a disulfide-linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa. (1,2) It is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. (3) CD69 is widely expressed on hematopoietic cells, including lymphocytes, neutrophils and eosinophils. (1-4) Although not detectable on resting lymphocytes, its expression is rapidly (within 2 hours) upregulated upon activation of T, B and NK cells, and neutrophils. (4) Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells. (4,5) MAb H1.2F3 augments PMA-induced T-cell proliferation,1,6 and induces redirected lysis of Fc receptor-bearing target cells by NK cells. (7)

Alternate Names

CD69, EA-1, Leu23, MLR-3, p60

Gene Symbol

CD69

Additional CD69 Products

Product Documents for CD69 Antibody (H1.2F3) [Alexa Fluor® 488]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CD69 Antibody (H1.2F3) [Alexa Fluor® 488]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD69 Antibody (H1.2F3) [Alexa Fluor® 488]

Customer Reviews for CD69 Antibody (H1.2F3) [Alexa Fluor® 488] (1)

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  • CD69 Antibody (H1.2F3) [Alexa Fluor® 488]
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Breast cancer tissue
    Species: Mouse
    Verified Customer | Posted 05/03/2019

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