Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Armenian Hamster IgG1 Clone # H1.2F3

Format

Azide and BSA Free
View all CD69 Primary Antibodies »

Product Specifications

Immunogen

Mouse dendritic epidermal cell line Y245

Reactivity Notes

Mouse reactivity reported in (PMID: 29367423).

Specificity

Mouse CD69/Very Early Activation Antigen (VEA), Mr 85 kDa (unreduced)

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG1

Scientific Data Images for CD69 Antibody (H1.2F3) - Azide and BSA Free

Flow Cytometry: CD69 Antibody (H1.2F3) - Azide and BSA Free [NBP1-28011]

Flow Cytometry: CD69 Antibody (H1.2F3) - Azide and BSA Free [NBP1-28011]

Flow Cytometry: CD69 Antibody (H1.2F3) [NBP1-28011] - PMA and Ionomycin stimulated BALB/c mouse splenocytes were stained with Hamster Anti-Mouse CD69-PACBLU.

Applications for CD69 Antibody (H1.2F3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000
Application Notes
Flow reactivity reported in (PMID: 29367423).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.1M BBS (pH 8.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD69

CD69, also known as very early activation (VEA) antigen, is a disulfide-linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa. (1,2) It is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. (3) CD69 is widely expressed on hematopoietic cells, including lymphocytes, neutrophils and eosinophils. (1-4) Although not detectable on resting lymphocytes, its expression is rapidly (within 2 hours) upregulated upon activation of T, B and NK cells, and neutrophils. (4) Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells. (4,5) MAb H1.2F3 augments PMA-induced T-cell proliferation,1,6 and induces redirected lysis of Fc receptor-bearing target cells by NK cells. (7)

Alternate Names

CD69, EA-1, Leu23, MLR-3, p60

Gene Symbol

CD69

Additional CD69 Products

Product Documents for CD69 Antibody (H1.2F3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD69 Antibody (H1.2F3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD69 Antibody (H1.2F3) - Azide and BSA Free

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