Cdc20 Antibody - BSA Free
Novus Biologicals | Catalog # NB100-59827
![Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59827]](https://resources.rndsystems.com/images/products/Cdc20-Antibody-Immunohistochemistry-NB100-59827-img0013.jpg "Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59827]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Porcine (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human cell division cycle 20 using the numbering given in entry NP_001246.2 (GeneID 991).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cdc20 Antibody - BSA Free
Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59827]
Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59827] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:500 (2ug/ml). Detection: DAB.![Immunoprecipitation: Cdc20 Antibody [NB100-59827]](https://resources.rndsystems.com/images/products/Cdc20-Antibody-Immunoprecipitation-NB100-59827-img0014.jpg "Immunoprecipitation: Cdc20 Antibody [NB100-59827]")
Immunoprecipitation: Cdc20 Antibody [NB100-59827]
Immunoprecipitation: Cdc20 Antibody [NB100-59827] - Detection of human CDC20 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDC20 antibody NB100-59827 (lot 2) used for IP at 6 ug/mg lysate. CDC20 was also immunoprecipitated by a previous lot (lot 1) of this antibody. For blotting immunoprecipitated CDC20, NB100-59827 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for Cdc20 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:200- 1:1000
Immunohistochemistry-Paraffin
1:200- 1:1000
Immunoprecipitation
2-10 ug/mg lysate
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Cdc20
Long Name
Cell division cycle protein 20 homolog
Alternate Names
p55CDC
Entrez Gene IDs
991 (Human)
Gene Symbol
CDC20
UniProt
Additional Cdc20 Products
Product Documents for Cdc20 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cdc20 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Cdc20 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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