Cdc23 Antibody

Novus Biologicals | Catalog # NB100-59829

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Cdc23 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 125 and 175 of human cell division cycle 23 (anaphase-promoting complex subunit 8) using the numbering given in entry NP_004652.2 (GeneID 8697).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cdc23 Antibody

Western Blot: Cdc23 Antibody [NB100-59829]

Western Blot: Cdc23 Antibody [NB100-59829]

Western Blot: Cdc23 Antibody [NB100-59829] - Detection of human and mouse CDC23/APC8 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC23/APC8 antibody NB100-59829 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Western Blot: Cdc23 Antibody [NB100-59829]

Western Blot: Cdc23 Antibody [NB100-59829]

Western Blot: Cdc23 Antibody [NB100-59829] - Detection of Human and Mouse CDC23/APC8 on HeLa whole cell lysate using NB100-59829. CDC23/APC8 was also immunoprecipitated by rabbit anti-CDC23/APC8 antibody NB100-59830.
Immunoprecipitation: Cdc23 Antibody [NB100-59829]

Immunoprecipitation: Cdc23 Antibody [NB100-59829]

Immunoprecipitation: Cdc23 Antibody [NB100-59829] - Detection of human CDC23/APC8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDC23/APC8 antibody NB100-59829 used for IP at 3 ug per reaction. CDC23/APC8 was also immunoprecipitated by another rabbit anti-CDC23/APC8 antibody. Detection: Chemiluminescence with an exposure time of 75 seconds.

Applications for Cdc23 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cdc23

CDC23/APC8 is a component of the APC/c (anaphase promoting complex/cyclosome) which is responsible for the ubiquitination and degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. The APC/c is composed of at least 11 subunits. Three subunits, CDC27 CDC16 and CDC23, contain a TPR (tetratricopeptide repeat) important for protein-protein interactions.

Alternate Names

ANAPC8anaphase promoting complex subunit 8, Anaphase-promoting complex subunit 8, APC8CDC23 (cell division cycle 23, yeast, homolog), cell division cycle 23 homolog (S. cerevisiae), cell division cycle protein 23 homolog, CUT23, Cyclosome subunit 8

Entrez Gene IDs

8697 (Human)

Gene Symbol

CDC23

UniProt

Q9UJX2 (Mouse)

Additional Cdc23 Products

Product Documents for Cdc23 Antibody

Certificate of Analysis

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Product Specific Notices for Cdc23 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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