CDC45L Antibody (4C2) - Azide and BSA Free

Novus Biologicals | Catalog # H00008318-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4C2

Format

Azide and BSA Free
View all CDC45L Primary Antibodies »

Product Specifications

Immunogen

CDC45L (NP_003495, 477 a.a. ~ 566 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FVCSTKNRRCKLLPLVMAAPLSMEHGTVTVVGIPPETDSSDRKNFFGRAFEKAAESTSSRMLHNHFDLSVIELKAEDRSKFLDALISLLS

Specificity

CDC45L - CDC45 cell division cycle 45-like (S. cerevisiae)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CDC45L Antibody (4C2) - Azide and BSA Free

Western Blot: CDC45L Antibody (4C2) [H00008318-M02]

Western Blot: CDC45L Antibody (4C2) [H00008318-M02]

Western Blot: CDC45L Antibody (4C2) [H00008318-M02] - CDC45L monoclonal antibody (M02), clone 4C2 Analysis of CDC45L expression in Jurkat.
Sandwich ELISA: CDC45L Antibody (4C2) [H00008318-M02] - Detection limit for recombinant GST tagged CDC45L is approximately 0.3ng/ml as a capture antibody.

Applications for CDC45L Antibody (4C2) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reviewed Applications

Read 1 review rated 3 using H00008318-M02 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CDC45L

The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported.

Alternate Names

CDC45 cell division cycle 45-like (S. cerevisiae), cell division cycle 45 homolog (S. cerevisiae), cell division cycle 45-like 2, human CDC45, S.cerevisiae, homolog)-like

Entrez Gene IDs

8318 (Human)

Gene Symbol

CDC45

OMIM

603465 (Human)

UniProt

NP_003495 (Human)

Additional CDC45L Products

Product Documents for CDC45L Antibody (4C2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CDC45L Antibody (4C2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CDC45L Antibody (4C2) - Azide and BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: HCT116 chromatin-enriched fraction, Sample Amount: 50 ug
    Species: Human
    Verified Customer | Posted 08/10/2010

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