CDT1 Antibody

Novus Biologicals | Catalog # NB100-1098

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CDT1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ARLAHQTRAEEGL corresponding to C-Terminus according to NP_112190.2.

Localization

Nuclear

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CDT1 Antibody

Western Blot: CDT1 Antibody [NB100-1098]

Western Blot: CDT1 Antibody [NB100-1098]

Western Blot: CDT1 Antibody [NB100-1098] - NB100-1098 staining of human brain lysate (RIPA buffer, 35 ug total protein per lane). Primary antibody (1 ug/ml) incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for CDT1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in human ovary lysates (calculated MW of 60.4 kDa band according to NP_112190.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CDT1

The CDT1 protein cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. It also binds DNA in a sequence-, strand-, and conformation-independent manner. CDT1 has been shown to interact with Geminin which inhibits both binding of the MCM complex to origins of replication and DNA-binding activity. There is some research that suggests CDT1 is a potential oncogene.

Alternate Names

chromatin licensing and DNA replication factor 1, DNA replication factor Cdt1, Double parked homolog, Double parked, Drosophila, homolog of, DUPRIS2

Entrez Gene IDs

81620 (Human)

Gene Symbol

CDT1

UniProt

NP_112190. (Human)

Additional CDT1 Products

Product Documents for CDT1 Antibody

Certificate of Analysis

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Product Specific Notices for CDT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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