Why Use CellSperse?
DIVAA CellSperse is a neutral metalloprotease from Bacillus polymyxa that promotes BME digestion and gentle cell dissociation for angiogenesis quantification in the Directed In Vivo Angiogenesis Assay (DIVAA).
For research use only. Not for diagnostic use.
Citation for CellSperse
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Extracellular Matrix composition modulates PDAC parenchymal and stem cell plasticity and behavior through the secretome
Authors: G Biondani, K Zeeberg, MR Greco, S Cannone, I Dando, ED Pozza, M Mastrodona, S Forciniti, V Casavola, M Palmieri, SJ Reshkin, RA Cardone
FEBS J., 2018;0(0):. 2018
Can cells grown in Basement Membrane Extract (BME), such as Catalog # 3433-005-01, be used in Flow Cytometry?
For preparation of a single cell suspension for applications such as FACS and re-culturing, digestion of the matrix and cell-cell bonds may be facilitated using enzymes such as Trypsin or Dispase. Our CellSperse, cat# 3450-048-05, is similar to Dispase, and can also be used for this purpose. However, some markers may be susceptible to degradation from CellSperse treatment. It is recommended to consult the literature for design of experiments for the particular cell type and markers of interest.
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