Chitotriosidase/CHIT1 Antibody

Novus Biologicals | Catalog # NBP1-97774

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence EHGPSPGQDTFCQGK corresponding to internal region (near C-Terminus) according to NP_003456.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Chitotriosidase/CHIT1 Antibody

Western Blot: Chitotriosidase/CHIT1 Antibody [NBP1-97774]

Western Blot: Chitotriosidase/CHIT1 Antibody [NBP1-97774]

Western Blot: CHIT1 Antibody [NBP1-97774] - (1ug/ml) Analysis of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Chitotriosidase/CHIT1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:2000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 48 kDa band observed in Daudi lysates (calculated MW of 51.7 kDa band according to NP_003456.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Chitotriosidase/CHIT1

Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. [provided by RefSeq]

Alternate Names

CHI3, CHIT1

Entrez Gene IDs

1118 (Human)

Gene Symbol

CHIT1

Additional Chitotriosidase/CHIT1 Products

Product Documents for Chitotriosidase/CHIT1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Chitotriosidase/CHIT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Chitotriosidase/CHIT1 Antibody

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