Ciliobrevin A

Tocris Bioscience | Catalog # 4529

Hedgehog (Hh) pathway antagonist, inhibits ciliogenesis; also inhibits dynein
Tocris Bioscience
Discontinued Product
4529 has been discontinued. View all Hedgehog Signaling Inhibitors products.

Key Product Details

Description

Hedgehog (Hh) pathway antagonist, inhibits ciliogenesis; also inhibits dynein

Alternative Names

HPI-4
View all Hedgehog Signaling Inhibitors »

Product Description

Ciliobrevin A is a Hedgehog (Hh) pathway antagonist; blocks Sonic hedgehog (Shh)-induced Hh pathway activation (IC50 = 7 μM) downstream of Smo. Perturbs primary cilia formation; inhibits cytoplasmic AAA+ ATPase dynein-dependent microtubule gliding and ATPase activity.

Product Specifications for Ciliobrevin A

Molecular Weight

358.18

Formula

C17H9Cl2N3O2

Storage

Store at +4°C

Purity

≥98% (HPLC)

Chemical Name

2,4-Dichloro-α-(3,4-4-oxo-2(1H)-quinazolinylidene)-β-oxobenzenepropanenitrile

CAS Number

302803-72-1

PubChem ID

6883982

InChI Key

SESYPWCSIZUIAS-FOWTUZBSSA-N

SMILES

O=C(C1=CC=CC=C1N2)N/C2=C(C(C3=C(Cl)C=C(Cl)C=C3)=O)\C#N

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 35.82 100

Preparing Stock Solutions for Ciliobrevin A

The following data is based on the product molecular weight 358.18.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.79 mL 13.96 mL 27.92 mL
5 mM 0.56 mL 2.79 mL 5.58 mL
10 mM 0.28 mL 1.40 mL 2.79 mL
50 mM 0.06 mL 0.28 mL 0.56 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 16 publications citing the usage of this product.

Product Documents for Ciliobrevin A

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Ciliobrevin A

For research use only

Customer Reviews for Ciliobrevin A (1)

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  • Effectively inhibits Hh signaling
    Name: Kishor Pant
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HepG2, and Huh,
    Verified Customer | Posted 02/10/2023
    Tested different concentrations and times for each cell for cilia imaging.
    This effectively inhibits Hh signaling. At 1 uM concentrations, we had satisfactory results with this compound.
    Ciliobrevin A 4529

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