CNAP1 Antibody (5E0Q9)

Novus Biologicals | Catalog # NBP3-33550

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 5E0Q9
View all CNAP1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human CNAP1 (Q15021).

Sequence:
LDDTTLSGSDRNAHLNALKMNCYALIRLLESFETMASQTNLVDLDLGGKGKKARTKAAHGFDWEEERQPILQLLTQLLQLDIRHLWNHSIIEEEFVSLVTG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

157 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CNAP1 Antibody (5E0Q9)

CNAP1 Antibody (5E0Q9)

Western Blot: CNAP1 Antibody (5E0Q9) [NBP3-33550] -

Western Blot: CNAP1 Antibody (5E0Q9) [NBP3-33550] - Western blot analysis of lysates from HeLa cells, using at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.
CNAP1 Antibody (5E0Q9)

Western Blot: CNAP1 Antibody (5E0Q9) [NBP3-33550] -

Western Blot: CNAP1 Antibody (5E0Q9) [NBP3-33550] - Western blot analysis of various lysates, using at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.

Applications for CNAP1 Antibody (5E0Q9)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CNAP1

CNAP1/hCAP-D2 is a regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condensed chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. It may target the condensin complex to DNA via its C-terminal domain (referenced from Swissprot).

Alternate Names

CAPD2, CAP-D2, Chromosome condensation-related SMC-associated protein 1, Chromosome-associated protein D2, CNAP1XCAP-D2 homolog, hCAP-D2condensin complex subunit 1, KIAA0159chromosome condensation related SMC associated protein 1, Non-SMC condensin I complex subunit D2, non-SMC condensin I complex, subunit D2

Gene Symbol

NCAPD2

Additional CNAP1 Products

Product Documents for CNAP1 Antibody (5E0Q9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CNAP1 Antibody (5E0Q9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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