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Recombinant Human COL4A3BP His Protein

Novus Biologicals | Catalog # NBP1-44465

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all COL4A3BP Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 347-598 of Human COL4A3BP

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMLH WPTSLPSGDA FSSVGTHRFV QKVEEMVQNH MTYSLQDVGG DANWQLVVEE GEMKVYRREV EENGIVLDPL KATHAVKGVT GHEVCNYFWN VDVRNDWETT IENFHVVETL ADNAIIIYQT HKRVWPASQR DVLYLSVIRK IPALTENDPE TWIVCNFSVD HDSAPLNNRC VRAKINVAMI CQTLVSPPEG NQEISRDNIL CKITYVANVN PGGWAPASVL RAVAKREYPK FLKRFTSYVQ EKTAGKPILF

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

32.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human COL4A3BP His Protein

SDS-PAGE: Recombinant Human COL4A3BP His Protein [NBP1-44465]

SDS-Page: COL4A3BP Protein [NBP1-44465] - CERT, 33.1 kDa (290aa) confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation, and Storage

NBP1-44465

Formulation	20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1 M NaCl
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: COL4A3BP

CERT is a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Recombinant human CERT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

Ceramide transfer protein, CERTFLJ20597, CERTL, collagen type IV alpha-3-binding protein, collagen, type IV, alpha 3 (Goodpasture antigen) binding protein, Goodpasture antigen-binding protein, GPBPSTARD11ceramide transporter, hCERT, lipid-transfer protein CERTL, StARD11, StAR-related lipid transfer (START) domain containing 11, StAR-related lipid transfer protein 11, START domain containing 11, START domain-containing protein 11

Gene Symbol

CERT1

Additional COL4A3BP Products

Product Documents for Recombinant Human COL4A3BP His Protein

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Product Specific Notices for Recombinant Human COL4A3BP His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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