CometChip Starter Kit

Catalog # Availability Size / Price Qty
4260-096-CSK
Product Details
FAQs
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CometChip Starter Kit Summary

Includes one CometChip Reagent Kit and the 96-Well CometChip System (macrowell former).

Key Benefits

• Single cell capture – no overlapping comets
• Multiple experiments on single slide
• Optimized electrophoresis conditions
• Parallel Processing and ease of handling
• Ready to use low melt agarose (LMAgarose) and Lysis Solution in convenient size

What is the CometChip System?

CometChip System is a high through-put 96 well platform to treat, electrophorese and measure DNA damage, in one or more cell types, on a single slide using the comet assay. The CometChip is an array of spatially encoded micropores patterned on agarose. 96 separate macrowells, each containing ~400 micropores, are created by inserting the CometChip into a magnetically sealable cassette. Cells added to each macrowell are deposited by gravity into the micropores - creating a pattern of non-overlapping cells.

Using the CometChip System, multiple experimental conditions are performed and evaluated in parallel by the addition of different chemicals to respective wells. Once treatment is complete and the CometChip removed from the magnetic cassette, an LMAgarose overlay is added to immobilize the cells within the micropores. When performing the Alkaline CometAssay, the CometChip is immersed in Lysis Buffer for gentle cellular lysis, followed by Alkaline Solution to unwind and denature the DNA and hydrolyze sites of damage. Comets are developed by alkaline electrophoresis using the CometAssay Electrophoresis System. Subsequent staining with SYBR® Gold, a fluorescent DNA intercalating dye, allows visualization of the comet tail shape for assessment of DNA damage by fluorescence microscopy.

Kit Contents

• CometAssay Lysis Solution
• CometAssay LMAgarose
• CometChip, 30 micron
• 96-Well CometChip System

Kit Contents

  • CometAssay Lysis Solution
  • CometAssay LMAgarose
  • CometChip, 30 micron
  • 96-Well CometChip System

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
N/A

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

Other Reagents Provided

FAQs

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