CometChip Starter Kit
CometChip Starter Kit Summary
• Single cell capture – no overlapping comets
• Multiple experiments on single slide
• Optimized electrophoresis conditions
• Parallel Processing and ease of handling
• Ready to use low melt agarose (LMAgarose) and Lysis Solution in convenient size
What is the CometChip System?
CometChip System is a high through-put 96 well platform to treat, electrophorese and measure DNA damage, in one or more cell types, on a single slide using the comet assay. The CometChip is an array of spatially encoded micropores patterned on agarose. 96 separate macrowells, each containing ~400 micropores, are created by inserting the CometChip into a magnetically sealable cassette. Cells added to each macrowell are deposited by gravity into the micropores - creating a pattern of non-overlapping cells.
Using the CometChip System, multiple experimental conditions are performed and evaluated in parallel by the addition of different chemicals to respective wells. Once treatment is complete and the CometChip removed from the magnetic cassette, an LMAgarose overlay is added to immobilize the cells within the micropores. When performing the Alkaline CometAssay, the CometChip is immersed in Lysis Buffer for gentle cellular lysis, followed by Alkaline Solution to unwind and denature the DNA and hydrolyze sites of damage. Comets are developed by alkaline electrophoresis using the CometAssay Electrophoresis System. Subsequent staining with SYBR® Gold, a fluorescent DNA intercalating dye, allows visualization of the comet tail shape for assessment of DNA damage by fluorescence microscopy.
• CometAssay Lysis Solution
• CometAssay LMAgarose
• CometChip, 30 micron
• 96-Well CometChip System
For research use only. Not for diagnostic use.
Can the CometChip® be stored before staining?
The neutralized gel can be stored in 20 mM Tris pH 7.4 buffer at 4 °C for a few days.
What is the size of a single pore in the CometChip®?
The CometChip micropores are 30 µm in diameter and 50 µm deep.
Is it possible to treat cells in the CometChip® for longer than the recommended time?
The protocol will be reflective of what we have validated in-house for best results. If longer treatment times are required, the treatment step should be carried out in a tissue culture plate, after which time the cells can be transferred to the CometChip.
Is it possible to reuse a CometChip® if not all wells are used?
The CometChip cannot be reused. After treatment and before lysis, the CometChip is covered with LMAgarose, which will fill the micropores in any unused wells.
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