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Product Documents for 96-Well CometChip System
Certificate of Analysis
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FAQs for 96-Well CometChip System
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Q: Can the CometChip® be stored before staining?
A: The neutralized gel can be stored in 20 mM Tris pH 7.4 buffer at 4 °C for a few days.
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Q: Is it possible to reuse a CometChip® if not all wells are used?
A: The CometChip cannot be reused. After treatment and before lysis, the CometChip is covered with LMAgarose, which will fill the micropores in any unused wells.
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Q: Is it possible to treat cells in the CometChip® for longer than the recommended time?
A: The protocol will be reflective of what we have validated in-house for best results. If longer treatment times are required, the treatment step should be carried out in a tissue culture plate, after which time the cells can be transferred to the CometChip.
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Q: What is the size of a single pore in the CometChip®?
A: The CometChip micropores are 30 µm in diameter and 50 µm deep.
-
Q: Can the CometChip® be stored before staining?
A: The neutralized gel can be stored in 20 mM Tris pH 7.4 buffer at 4 °C for a few days.
-
Q: Is it possible to reuse a CometChip® if not all wells are used?
A: The CometChip cannot be reused. After treatment and before lysis, the CometChip is covered with LMAgarose, which will fill the micropores in any unused wells.
-
Q: Is it possible to treat cells in the CometChip® for longer than the recommended time?
A: The protocol will be reflective of what we have validated in-house for best results. If longer treatment times are required, the treatment step should be carried out in a tissue culture plate, after which time the cells can be transferred to the CometChip.
-
Q: What is the size of a single pore in the CometChip®?
A: The CometChip micropores are 30 µm in diameter and 50 µm deep.
-
Q: Can the CometChip® be stored before staining?
A: The neutralized gel can be stored in 20 mM Tris pH 7.4 buffer at 4 °C for a few days.
-
Q: Is it possible to reuse a CometChip® if not all wells are used?
A: The CometChip cannot be reused. After treatment and before lysis, the CometChip is covered with LMAgarose, which will fill the micropores in any unused wells.
-
Q: Is it possible to treat cells in the CometChip® for longer than the recommended time?
A: The protocol will be reflective of what we have validated in-house for best results. If longer treatment times are required, the treatment step should be carried out in a tissue culture plate, after which time the cells can be transferred to the CometChip.
-
Q: What is the size of a single pore in the CometChip®?
A: The CometChip micropores are 30 µm in diameter and 50 µm deep.
-
Q: Can the CometChip® be stored before staining?
A: The neutralized gel can be stored in 20 mM Tris pH 7.4 buffer at 4 °C for a few days.
-
Q: Is it possible to reuse a CometChip® if not all wells are used?
A: The CometChip cannot be reused. After treatment and before lysis, the CometChip is covered with LMAgarose, which will fill the micropores in any unused wells.
-
Q: Is it possible to treat cells in the CometChip® for longer than the recommended time?
A: The protocol will be reflective of what we have validated in-house for best results. If longer treatment times are required, the treatment step should be carried out in a tissue culture plate, after which time the cells can be transferred to the CometChip.
-
Q: What is the size of a single pore in the CometChip®?
A: The CometChip micropores are 30 µm in diameter and 50 µm deep.