CRANAD 2

Key Product Details

Product Description

Key information: CRANAD 2 is a curcumin derivatized near-infrared probe that binds to Aβ40 aggregates. Penetrates the blood-brain barrier.

Used for: amyloid- detection in vitro, tissue and in vivo. Shown to bind to plaques in APP-PS1 transgenic mice, in vitro. Detects senile plaques in 19-month-old Tg2576 mice in vivo.

Application: Fluorescence microscopy, epifluorescence microscopy.

Properties and Photophysical Data: CRANAD 2 binds to Aβ40 aggregates (K_d = 38 nM) and elicits upon interacting with Aβ aggregates, an emission blue shift (from 805 nm to 715 nm). When unbound in PBS, excitation and emission maxima (λ) are 640 nm and 805 nm, respectively.

Product Specifications for CRANAD 2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for CRANAD 2

The following data is based on the product molecular weight 410.26.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.05 mM	48.75 mL	243.75 mL	487.50 mL
0.25 mM	9.75 mL	48.75 mL	97.50 mL
0.5 mM	4.87 mL	24.37 mL	48.75 mL
2.5 mM	0.97 mL	4.87 mL	9.75 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Ran Non-conjugated small molecule FRET for differentiating monomers from higher molecular weight amyloid beta species. PLoS One. 2011 PMID: 21559413
• Ni In-vitro and in-vivo characterization of CRANAD-2 for multi-spectral optoacoustic tomography and fluorescence imaging of amyloid-beta deposits in Alzheimer mice. Photoacoustics 2021 PMID: 34354924
• Ran Design, synthesis, and testing of difluoroboron-derivatized curcumins as near-infrared probes for in vivo detection of amyloid-beta deposits. J.Am.Chem.Soc. 2009 PMID: 19807070

Product Documents for CRANAD 2