Cultrecoat In Vitro Vascular Permeability Assay, 24-well

Discontinued Product

3475-024-K has been discontinued.
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Product Details
Citations (2)
FAQs
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Cultrecoat In Vitro Vascular Permeability Assay, 24-well Summary

Acelerates the screening process for signal transduction pathway modulators and compounds that influence vascular permeability.

Why Use Cultrecoat In Vitro Vascular Permeability Assay, 24-well?

The CultreCoat Vascular Permeability Assay employs a simplified Boyden chamber design with a one micron polyethylene terephthalate (PET) membrane coated with collagen I. Ports within the migration chamber (top) allow access to the assay chamber (bottom) without dismantling the device. This design is easier to use, prevents contamination, and is adaptable for robotic high throughput systems. The assay chamber may be directly analyzed in a plate reader, eliminating transfer steps that introduce additional variability to the assay. The CultreCoat 24 well Vascular Permeability Assay accelerates the screening process for signal transduction pathway modulators and compounds that influence vascular permeability, offering a flexible, standardized, high-throughput format for quantifying the degree to which genes or compounds can influence the maintenance of endothelial cell-to-cell adhesion. The CultreCoat 24 well Vascular Permeability Assays are provided as either a single 96 well plate providing capacity for large screening experiments or as 24 individual inserts providing flexibility for smaller studies. The CultreCoat Vascular Permeability Assay employs a simplified Boyden chamber design with a one micron polyethylene terephthalate (PET) membrane coated with collagen I. Ports within the migration chamber (top) allow access to the assay chamber (bottom) without dismantling the device. This design is easier to use, prevents contamination, and is adaptable for robotic high throughput systems. The assay chamber may be directly analyzed in a plate reader, eliminating transfer steps that introduce additional variability to the assay.

Kit Contents

• CultreCoat® 24 Well Vascular Permeability Chamber
• CultreCoat® FITC-Dextran

Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.

Specifications

Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

Citations for Cultrecoat In Vitro Vascular Permeability Assay, 24-well

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The involvement of autotaxin in renal interstitial fibrosis through regulation of fibroblast functions and induction of vascular leakage
    Authors: N Sakai, G Bain, K Furuichi, Y Iwata, M Nakamura, A Hara, S Kitajima, A Sagara, T Miyake, T Toyama, K Sato, S Nakagawa, M Shimizu, S Kaneko, T Wada
    Sci Rep, 2019;9(1):7414.  2019
  2. Spleen-derived classical monocytes mediate lung ischemia-reperfusion injury through IL-1?
    Authors: HM Hsiao, R Fernandez, S Tanaka, W Li, JH Spahn, S Chiu, M Akbarpour, D Ruiz-Perez, Q Wu, C Turam, D Scozzi, T Takahashi, HP Luehmann, V Puri, GRS Budinger, AS Krupnick, AV Misharin, KJ Lavine, Y Liu, AE Gelman, A Bharat, D Kreisel
    J. Clin. Invest., 2018;128(7):2833-2847.  2018

FAQs

  1. Is it possible to run less than 24 wells at once?

    • Yes, its possible to run less than 24 wells at once.  Unused inserts should be stored at 4 degrees C with dessicant until needed.

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