Cultrex 3-D Culture Cell Harvest Buffers
Cultrex 3-D Culture Cell Harvest Buffers SummaryOptimized and standardized solutions for the isolation of intact 3-D structures from Basement Membrane Extract (BME) or Laminin I hydrogels. This product is also a part of the Cultrex 3-D Culture Cell Harvesting Kit.
Why Use Cultrex 3-D Culture Cell Harvest Buffers?
3-D Cultures exhibit cellular behaviors and morphologies similar to those seen in vivo; however, the adaptation of these models for studying biochemical processes has been impeded by the challenge of separating intact cells from extracellular matrix hydrogels. Commonly, proteases are employed to degrade these extracellular proteins; however, proteases also degrade proteins on the cell surface and may carry over into lysate preparations. Non-enzymatic methodologies have also been described for depolymerizing extracellular matrix proteins, although the implementation of these protocols remains problematic for some researchers. The Cultrex 3-D Culture Cell Harvest Buffers depolymerize BME and Laminin I hydrogels, non-enzymatically, providing an optimized and standardized solution for the isolation of 3-D structures.
• 3-D Culture 10X Cell Harvesting Buffer
• 3-D Culture 10X Cell Wash Buffer
For research use only. Not for diagnostic use.
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