Cultrex 3-D Culture Cell Harvest Buffers

Discontinued Product

3448-020-CH has been discontinued.
View all Basement Membrane Extracts products.
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Cultrex 3-D Culture Cell Harvest Buffers Summary

Optimized and standardized solutions for the isolation of intact 3-D structures from Basement Membrane Extract (BME) or Laminin I hydrogels. This product is also a part of the Cultrex 3-D Culture Cell Harvesting Kit.

Why Use Cultrex 3-D Culture Cell Harvest Buffers?

3-D Cultures exhibit cellular behaviors and morphologies similar to those seen in vivo; however, the adaptation of these models for studying biochemical processes has been impeded by the challenge of separating intact cells from extracellular matrix hydrogels.  Commonly, proteases are employed to degrade these extracellular proteins; however, proteases also degrade proteins on the cell surface and may carry over into lysate preparations. Non-enzymatic methodologies have also been described for depolymerizing extracellular matrix proteins, although the implementation of these protocols remains problematic for some researchers. The Cultrex 3-D Culture Cell Harvest Buffers depolymerize BME and Laminin I hydrogels, non-enzymatically, providing an optimized and standardized solution for the isolation of 3-D structures.

Kit Contents

• 3-D Culture 10X Cell Harvesting Buffer
• 3-D Culture 10X Cell Wash Buffer


Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended on the label.
Store the unopened product at room temperature.


For research use only. Not for diagnostic use.

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3-D Culture Cell Harvest Buffers
By Leslie Priddy on 04/01/2018
Application: Immunoassay Standard