Cultrex 3-D Culture Cell Harvesting Kit
Discontinued Product
Cultrex 3-D Culture Cell Harvesting Kit Summary
Optimized and standardized kit for the isolation of intact 3-D structures from Basement Membrane Extract (BME) Matrix or Laminin I hydrogels and preparation of cell lysates for subsequent biochemical analysis.Why Use Cultrex 3-D Culture Cell Harvesting Kit?
3-D Cultures exhibit cellular behaviors and morphologies similar to those seen in vivo; however, the adaptation of these models for studying biochemical processes has been impeded by the challenge of separating intact cells from extracellular matrix hydrogels. Commonly, proteases are employed to degrade these extracellular proteins; however, proteases also degrade proteins on the cell surface and may carry over into lysate preparations. Non-enzymatic methodologies have also been described for depolymerizing extracellular matrix proteins, although the implementation of these protocols remains problematic for some researchers. The Cultrex 3-D Culture Cell Harvesting Kit depolymerizes BME and Laminin I hydrogels, non-enzymatically, providing an optimized and standardized solution for the isolation of 3-D structures and preparation of cell lysates for subsequent biochemical analysis.
Kit Contents
• 3-D Culture 10X Cell Harvesting Buffer
• 3-D Culture 10X Cell Wash Buffer
• 3-D Culture Sample Buffer
• 3-D Culture 10X Loading Buffer
• Human Anti-G3PDH Antibody
Specifications
Limitations
For research use only. Not for diagnostic use.
Product Datasheets
Citation for Cultrex 3-D Culture Cell Harvesting Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Cancer-associated fibroblasts suppress SOX2-induced dysplasia in a lung squamous cancer coculture
Authors: S Chen, A Giannakou, S Wyman, J Gruzas, J Golas, W Zhong, C Loreth, L Sridharan, TT Yamin, M Damelin, KG Geles
Proc. Natl. Acad. Sci. U.S.A., 2018;115(50):E11671-E11680. 2018
FAQs
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How can I harvest my organoids or 3-D cultured cells for subsequent analysis?
Organoids or 3-D cultured cells may be harvested using the Cultrex® 3-D Culture Cell Harvesting Kit (Catalog # 3448-020-K)
Reviews for Cultrex 3-D Culture Cell Harvesting Kit
Average Rating: 5 (Based on 4 Reviews)
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Reason for Rating: Used to digest Organoids to make Cell lysates for Western Blot to look at protein expression.
