Basement membrane extract (BME) is a type of extracellular matrix (ECM) derived from Engelbreth-Holm-Swarm mouse sarcomas. BME is commonly used in cell culture and tissue engineering to provide a 2-D or 3-D scaffold for cells, allowing them to grow, differentiate, and interact in an environment that closely mimics the native ECM. This makes BME invaluable for modeling the behavior of cells in vivo. All Cultrex BME formulations are rigorously tested to ensure performance, quality, and lot-to-lot consistency.
Cultrex 3-D Spheroid Fluorometric Assay, 96-well
R&D Systems | Catalog # 3510-096-K
Fluorometric Proliferation/Viability Assay
Discontinued Product
3510-096-K has been discontinued.
View all Basement Membrane Extracts products.
Key Product Details
Features
Provides a powerful tool for modeling tumor growth response in vitro using fluorometric detection.
Species
Mouse
Product Summary for Cultrex 3-D Spheroid Fluorometric Assay, 96-well
Why Use Cultrex 3-D Spheroid Fluorometric Assay, 96-well?
The Cultrex 3-D Spheroid Fluorometric Proliferation/Viability Assay utilizes a 3-D Culture Qualified 96 Well Spheroid Formation Plate alongside a specialized Spheroid Formation ECM to drive aggregation and/or spheroid formation of cells. Upon completion of spheroid formation, the spheroid may be treated with pharmacological agents to evaluate tumor viability after drug treatment. Tumor spheroid expansion is visualized microscopically and can be quantitated through image analysis software for real-time and label free evaluation. At the conclusion of the assay, cell viability may be assessed by fluorescence using Resazurin. The 3-D Spheroid Fluorometric Proliferation/Viability Assay offers an in vitro, standardized, three-dimensional, high content format for inducing multicellular tumor spheroid (MCTS) formation and quantifying cell viability within the spheroids in response to pharmacological treatment.
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Kit Contents for Cultrex 3-D Spheroid Fluorometric Assay, 96-well
- Cultrex Spheroid Formation Matrix
- 3-D Culture Qualified 96 Well Spheroid Formation Plate with Strip Seals
- Resazurin
Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.
Formulation, Preparation, and Storage
Shipping
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: Basement Membrane Extracts
Additional Basement Membrane Extracts Products
Product Documents for Cultrex 3-D Spheroid Fluorometric Assay, 96-well
Certificate of Analysis
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Citations for Cultrex 3-D Spheroid Fluorometric Assay, 96-well
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