Cultrex Stem Cell Qualified Human Vitronectin, PathClear

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Catalog # Availability Size / Price Qty
3421-001-03
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Cultrex Stem Cell Qualified Human Vitronectin, PathClear Summary

Cultrex Stem Cell Qualified Human Vitronectin, Pathclear is a purified stromal extracellular matrix protein that provides a functionally defined and effective feeder-free surface for the attachment and maintenance of pluripotent stem cells in culture.

Key Benefits

• Qualified for pluripotent stem cell culture
• Supports robust, long term proliferation of iPSCs and ESCs
• Pathclear-tested to ensure product is free of human virus and pathogens
• Source tissue is LDEV-free

Why Use Cultrex Stem Cell Qualified Human Vitronectin?

Cultrex Stem Cell Qualified Human Vitronectin, Pathclear is purified from human plasma and is Pathclear certified to be free of common pathogens. Vitronectin is an extracellular, soluble, disulfide-linked dimer, composed of a 75 kDa and a 65 kDa peptide chain with a total molecular weight of 140 kDa. Vitronectin is a major plasma glycoprotein that promotes cellular adhesion and spreading. It also inhibits the membrane damaging effect of the terminal cytolytic complement pathway and binds to several serpin serine protease inhibitors. Vitronectin, along with collagen IV, fibronectin, and laminin can support robust, long term proliferation of undifferentiated human embryonic stem cells. Vitronectin can be used for coating tissue culture surfaces to promote cell adhesion, proliferation and differentiation, or as an additive for serum-free media. Vitronectin is purified from human plasma and is 0.2 μm sterile filtered.

Specifications

Source
Human plasma
Protein Concentration
1 mg/mL
Endotoxin Level
≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Testing Cell Culture
Supports the attachment of human iPSCs.

Effectively maintains human iPSCs in a pluripotent state in a feeder-free culture.
Viral Testing
Tested negative by PCR test for mycoplasma and human pathogenic viruses, including: EBV, HAdV, Hantaan, HCMV, Hepatitis A, Hepatitis B, Hepatitis C, HHV 6, HHV 8, HIV1, HIV2, HSV 1, HSV 2, HTLV 1, HTLV 2, LCMV, Seoul, Sin Nombre, and VZV.
Stability
Product is stable for a minimum of 3 months from date of shipment when stored at ≤ -20 °C. For optimal stability store at ≤ -70 °C. Avoid freeze-thaw cycles.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Species
Human

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

Other Reagents Provided

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