Cyanine 3, SE

Tocris Bioscience | Catalog # 5435

Orange fluorescent dye for labeling of amines; supplied as NHS ester
Tocris Bioscience
Discontinued Product
5435 has been discontinued. View all Fluorescent Dyes products.

Key Product Details

Description

Orange fluorescent dye for labeling of amines; supplied as NHS ester

Wavelength

Excitation = 550 nm, Emission = 570 nm
View all Fluorescent Dyes »

Product Description

Cyanine 3, SE is an orange fluorescent dye for labeling of amines. Emission maximum ~564 nm.

Optical Data

Cyanine 3, SE Dye Spectra
Emission Color Orange
Brightness
λabs 550 nm
λem 570 nm
Extinction Coefficient (ε) 150000 M-1cm-1
Quantum Yield (φ) 0.31
Closest Laser line 532 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable No
Correction Factor 260 0.04
Correction Factor 280 0.09
Application Flow Cytometry
Alternative For Alexa Fluor 546; DyLight 549

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Product Specifications for Cyanine 3, SE

Molecular Weight

727.84

Formula

C35H41N3O10S2

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

2-[3-[1-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1-propen-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium, inner salt

CAS Number

146368-16-3

PubChem ID

91811030

InChI Key

SOVMUUCAZOFIQP-UHFFFAOYSA-N

SMILES

CC[N+]1=C(C(C)(C)C2=C1C=CC(S(=O)([O-])=O)=C2)/C=C/C=C3N(CCCCCC(ON4C(CCC4=O)=O)=O)C(C=CC(S(=O)(O)=O)=C5)=C5C/3(C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMF 72.78 100
DMSO 72.78 100

Preparing Stock Solutions for Cyanine 3, SE

The following data is based on the product molecular weight 727.84.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.37 mL 6.87 mL 13.74 mL
5 mM 0.27 mL 1.37 mL 2.75 mL
10 mM 0.14 mL 0.69 mL 1.37 mL
50 mM 0.03 mL 0.14 mL 0.27 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Cyanine 3, SE

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Cyanine 3, SE

For research use only

Customer Reviews for Cyanine 3, SE (1)

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  • Fluorescent probe production and application
    Name: Vladimir Khayenko
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: Cortical neurons
    Verified Customer | Posted 03/20/2020
    The Sulfo-Cyanine dye was conjugated to bioactive peptide that binds gephyrin. The labeling of gephyrin was done in Cortical neurons (DIV21) infected with lentivirus to express mEos2-Gephyrin.
    Cyanine 3, SE 5435

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