Cyanine 3 Tyramide

Tocris Bioscience | Catalog # 6457

Orange fluorescent reagent widely used for signal amplification in IHC and FISH
Tocris Bioscience

Key Product Details

Description

Orange fluorescent reagent widely used for signal amplification in IHC and FISH

Wavelength

Excitation = 550 nm, Emission = 563 nm
View all Tyramide Signaling Amplification/TSA »

Product Description

Cyanine 3 Tyramide is an orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.

Optical Data

Cyanine 3 Tyramide Dye Spectra
Emission Color Orange
λabs 550 nm
λem 563 nm
Extinction Coefficient (ε) 150000 M-1cm-1
Quantum Yield (φ) 0.31
Closest Laser line 532 nm
Application Tyramide Signal Amplification (TSA) in IHC, ICC, ISH, FISH

Spectra Viewer

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Product Specifications for Cyanine 3 Tyramide

Molecular Weight

749.94

Formula

C39H47N3O8S2

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

2-[3-[(1-Ethyl-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene)-1-propen-1-yl]-1-[6-[[2-(4-hydroxyphenyl)ethyl]amino-6-oxohexyl]-3,3-dimethyl-5-sulfo-3H-indolium inner salt

CAS Number

174961-75-2

InChI Key

JPXIOSIRIWBDIC-UHFFFAOYSA-N

SMILES

CCN1C2=C(C=C(C=C2)S(O)(=O)=O)C(/C1=C\C=C\C3=[N+](CCCCCC(NCCC4=CC=C(O)C=C4)=O)C5=C(C=C(C=C5)S([O-])(=O)=O)C3(C)C)(C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 12 16

Preparing Stock Solutions for Cyanine 3 Tyramide

The following data is based on the product molecular weight 749.94.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.16 mM 8.33 mL 41.67 mL 83.34 mL
0.8 mM 1.67 mL 8.33 mL 16.67 mL
1.6 mM 0.83 mL 4.17 mL 8.33 mL
8 mM 0.17 mL 0.83 mL 1.67 mL

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Product Documents for Cyanine 3 Tyramide

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Cyanine 3 Tyramide

For research use only

Citations for Cyanine 3 Tyramide

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