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Cyanine 5, SE

Catalog #: 5436 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
5436/10
Cyanine 5, SE | CAS No. 146368-14-1 | Fluorescent Dyes
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Description: Red fluorescent dye for the labeling of amines; supplied as NHS ester
Alternative Names: Cy5 NHS Ester

Chemical Name: 2-[5-[1-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium, inner salt

Purity: ≥95%

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Biological Activity

Cyanine 5, SE is a red fluorescent dye for the labeling of amines. Excitation maximum ~649 nm; emission maximum ~666 nm.

For more information on how Cyanine 5, SE may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes.

Optical Data for Cyanine 5, SE

Cyanine 5, SE Dye Spectra
Emission Color Red
Brightness
λabs 649 nm
λem 666 nm
Extinction Coefficient (ε) 250000 M-1cm-1
Quantum Yield (φ) 0.2
Closest Laser line 647 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable No
Correction Factor 260 0.03
Correction Factor 280 0.04
Alternative For Alexa Fluor 647; DyLight 649

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

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Technical Data

M.Wt:
753.88
Formula:
C37H43N3O10S2
Solubility:
Soluble to 100 mM in DMSO and to 100 mM in DMF
Purity:
≥95%
Storage:
Store at -20°C
CAS No:
146368-14-1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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