Cyanine 5, SE
Tocris Bioscience | Catalog # 5436
Key Product Details
Description
Alternative Names
Wavelength
Product Description
Key information:Cyanine 5, SE is a widely used red fluorescent dye supplied with an NHS ester reactive group for the labeling of primary amines.
Application: Suitable for fluorescence microscopy, confocal microscopy and flow cytometry.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 649 nm and 666 nm, respectively; quantum yield = 0.2; extinction coefficient = 250,000 M-1cm-1; A280 correction factor = 0.04.
For more information on how Cyanine 5, SE may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes.
Optical Data
| Emission Color | Red |
| Brightness | |
| λabs | 649 nm |
| λem | 666 nm |
| Extinction Coefficient (ε) | 250000 M-1cm-1 |
| Quantum Yield (φ) | 0.2 |
| Closest Laser line | 647 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Correction Factor 260 | 0.03 |
| Correction Factor 280 | 0.04 |
| Application | Fluorescence Microscopy, Confocal Microscopy, Flow cytometry |
| Alternative For | Alexa Fluor® 647; DyLight™ 649 |
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Product Specifications for Cyanine 5, SE
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMF | 75.39 | 100 | |
| DMSO | 75.39 | 100 |
Preparing Stock Solutions for Cyanine 5, SE
The following data is based on the product molecular weight 753.88.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.33 mL | 6.63 mL | 13.26 mL |
| 5 mM | 0.27 mL | 1.33 mL | 2.65 mL |
| 10 mM | 0.13 mL | 0.66 mL | 1.33 mL |
| 50 mM | 0.03 mL | 0.13 mL | 0.27 mL |
Calculators
Background References
References are publications that support the biological activity of the product. See our Citations tab to view 4 publications citing the usage of this product.
- Shin Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters. Nucleic Acids Res. 2014 PMID: 24753407
Product Documents for Cyanine 5, SE
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cyanine 5, SE
For research use only
Citations for Cyanine 5, SE
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