Cynomolgus Monkey 20S Immunoproteasome Protein, CF

Catalog # Availability Size / Price Qty
E-378-025
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Cynomolgus Monkey 20S Immunoproteasome Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
The 20S Proteasome is able to degrade substrates in an ATP-independent manner.  It can be activated chemically with SDS (0.035%, final) or by the addition of PA28.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial 20S Proteasome concentration of 0.5 - 5.0 nM.
Source
Cynomolgus monkey spleen-derived 20S Immunoproteasome protein
Predicted Molecular Mass
700 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-378

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.6, 100 mM NaCl, 1 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: 20S Immunoproteasome

The 20S Immunoproteasome is a modified form of the constitutively active 20S Proteasome core particle, and is the catalytic subunit of the multi-complex Immunoproteasome.  The structure of the 20S Immunoproteasome is similar to the 20S Proteasome, which is composed of 28 non-identical subunits arranged in four stacked rings.  During 20S Immunoproteasome  assembly in humans, three catalytic beta subunits ( beta 1, beta 2, and beta 5) in the two interior rings of the 20S Proteasome are replaced by three IFN gamma -inducible subunits: beta 1i/LMP2( PSMB9), beta 2i/MECL-1 (PSMB10), and beta 5i/LMP7 (PSMB8).  In their mature forms these subunits are highly conserved in C. fascicularis, with identities of 97%, 91%, and 98%, respectively.  The 20S Immunoproteasome is commonly associated with the 19S, PA28 alpha / beta or PA28 gamma regulatory complexes.  20S Immunoproteasome expression is elevated in antigen presenting cells of the immune system where the Proteasome activity selectively degrades intacellular proteins in a manner that optimizes the generation of peptides for MHC class I antigen presentation.  Selective inhibition of 20S Immunoproteasome proteolytic activity using small molecule inhibitors is being extensively examined for therapeutic intervention in cancer and inflammatory diseases.  This protein has been purified from spleen cells collected from the crab-eating macaque, Cynomolgus fascicularis (cyno).

References
  1. Kim, H.M. et al. (2011) Biochim Biophys. Acta  1809: 67
  2. Xie, Y. (2010) J. Mol. Cell Biol.  2: 308
  3. Kloetzel, P.M. (2001) Nat. Rev. Mol. Cell Biol.  2: 179
  4. Stadtmueller, B.M. & Hill, C.P. (2011) Mol. Cell  41: 8
  5. Cascio, P. et al. (2001) EMBO J.  20: 2357
Alternate Names
20S Immunoproteasome

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