Cytochrome P450 2E1 Antibody (M12P4H2)

Novus Biologicals | Catalog # NBP3-07825

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # M12P4H2
View all Cytochrome P450 2E1 Primary Antibodies »

Product Specifications

Immunogen

Ovalbumin-conjugated synthetic peptide HIGFGCIPPR (C-terminal sequence) (Uniprot: P05181)

Localization

Endoplasmic reticulum membrane, Microsome membrane

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1mg/ml. (NBP3-08831)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytochrome P450 2E1 Antibody (M12P4H2)

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (M12P4H2) [NBP3-07825]

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (M12P4H2) [NBP3-07825]

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (M12P4H2) [NBP3-07825] - Formalin-fixed, paraffin-embedded human Hepatic Carcinoma stained with Cytochrome P450 2E1 Mouse Monoclonal Antibody (M12P4H2).

Applications for Cytochrome P450 2E1 Antibody (M12P4H2)

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.1-0.2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytochrome P450 2E1

Alternate Names

4-nitrophenol 2-hydroxylase, CPE1, CYP2Ecytochrome P450 2E1, CYPIIE1, cytochrome P450, family 2, subfamily E, polypeptide 1, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1, Cytochrome P450-J, EC 1.14.13.-, EC 1.14.13.n7, EC 1.14.14.1, flavoprotein-linked monooxygenase, microsomal monooxygenase, P450C2E, P450-J, xenobiotic monooxygenase

Gene Symbol

CYP2E1

UniProt

P05181 (Human)

Additional Cytochrome P450 2E1 Products

Product Documents for Cytochrome P450 2E1 Antibody (M12P4H2)

Certificate of Analysis

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Product Specific Notices for Cytochrome P450 2E1 Antibody (M12P4H2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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