Cytokeratin 6a Antibody (KRT6A/2368)

Novus Biologicals | Catalog # NBP2-79719

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # KRT6A/2368
View all Cytokeratin 6a Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Cytokeratin 6aprotein (Uniprot: P02538)

Localization

Cytoplasmic

Marker

Basal Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11521)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Cytokeratin 6a Antibody (KRT6A/2368)

Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (KRT6A/2368) [NBP2-79719]

Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (KRT6A/2368) [NBP2-79719]

Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (KRT6A/2368) [NBP2-79719] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 6a Antibody (KRT6A/2368).
Flow Cytometry: Cytokeratin 6a Antibody (KRT6A/2368) [NBP2-79719]

Flow Cytometry: Cytokeratin 6a Antibody (KRT6A/2368) [NBP2-79719]

Flow Cytometry: Cytokeratin 6a Antibody (KRT6A/2368) [NBP2-79719] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 6a Antibody (KRT6A/2368)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (KRT6A/2368) [NBP2-79719]

Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (KRT6A/2368) [NBP2-79719]

Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (KRT6A/2368) [NBP2-79719] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Cytokeratin 6a Antibody (KRT6A/2368).
Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (KRT6A/2368) [NBP2-79719]

Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (KRT6A/2368) [NBP2-79719]

Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (KRT6A/2368) [NBP2-79719] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Cytokeratin 6a Antibody (KRT6A/2368).
Protein Array: Cytokeratin 6a Antibody (KRT6A/2368) [NBP2-79719]

Protein Array: Cytokeratin 6a Antibody (KRT6A/2368) [NBP2-79719]

Protein Array: Cytokeratin 6a Antibody (KRT6A/2368) [NBP2-79719] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 6a Antibody (KRT6A/2368). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for Cytokeratin 6a Antibody (KRT6A/2368)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 6a

The human type II Cytokeratin 6 (CK6; 56 kDa) is expressed in a heterogeneous array of epithelial tissues under normal conditions, but is better known for its strong induction in stratified epithelia that feature an enhanced cell proliferation rate or abnormal differentiation. It has been demonstrated that CK6 isoform, CK6a, is clearly the dominant CK6 isoform in skin tissue samples and cultured epithelial cell lines and that the various isoforms are differentially regulated within and between epithelial tissue types (1). The murine genome is known to have two keratin 6 (CK6) genes, mouse CK6, CK6a and MK6b. These genes display a complex expression pattern with constitutive expression in the epithelia of oral mucosa, hair follicles, and nail beds (2). Pachyonychia congenital (PC) is characterized by hypertrophic nail dystrophy and associated ectodermal features. PC-1 subtype is associated with mutations in keratins 6a or 16, whereas PC-2 subtype is linked to mutations in keratins 6b or 17 (3).

Alternate Names

Allergen Hom s 5, CK6A, CK-6A, CK6C, CK6D, CK-6D, cytokeratin 6A, cytokeratin 6C, cytokeratin 6D, Cytokeratin-6A, Cytokeratin-6D, K6A, K6C, K6D, keratin 6A, keratin 6C, keratin 6D, keratin, epidermal type II, K6A, keratin, type II cytoskeletal 6A, Keratin-6A, KRT6C, KRT6D, Type-II keratin Kb6

Gene Symbol

KRT6A

UniProt

P02538 (Human)

Additional Cytokeratin 6a Products

Product Documents for Cytokeratin 6a Antibody (KRT6A/2368)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 6a Antibody (KRT6A/2368)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Cytokeratin 6a Antibody (KRT6A/2368)

There are currently no reviews for this product. Be the first to review Cytokeratin 6a Antibody (KRT6A/2368) and earn rewards!

Have you used Cytokeratin 6a Antibody (KRT6A/2368)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies