DAAM1 Antibody (4H3) - Azide and BSA Free

Novus Biologicals | Catalog # H00023002-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA, Immunoprecipitation, Proximity Ligation Assay

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4H3

Format

Azide and BSA Free
View all DAAM1 Primary Antibodies »

Product Specifications

Immunogen

DAAM1 (NP_055807, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAPRKRGGRGISFIFCCFRNNDHPEITYRLRNDSNFALQTMEPALPMPPVEELDVMFSELVDELDLTDKHREAMFALPAEKKWQIYCSKKKDQEENKGATSWPEFYIDQL

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29972794).

Specificity

DAAM1 (4H3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for DAAM1 Antibody (4H3) - Azide and BSA Free

Western Blot: DAAM1 Antibody (4H3) [H00023002-M03]

Western Blot: DAAM1 Antibody (4H3) [H00023002-M03]

Western Blot: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of DAAM1 expression in A-431 (Cat # L015V1).
Western Blot: DAAM1 Antibody (4H3) [H00023002-M03]

Western Blot: DAAM1 Antibody (4H3) [H00023002-M03]

Western Blot: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of DAAM1 expression in PC-12 (Cat # L012V1).
Western Blot: DAAM1 Antibody (4H3) [H00023002-M03]

Western Blot: DAAM1 Antibody (4H3) [H00023002-M03]

Western Blot: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of DAAM1 expression in transfected 293T cell line by DAAM1 monoclonal antibody (M03), clone 4H3. Lane 1: DAAM1 transfected lysatE (122.3 KDa). Lane 2: Non-transfected lysate.
ELISA: DAAM1 Antibody (4H3) [H00023002-M03]

ELISA: DAAM1 Antibody (4H3) [H00023002-M03]

ELISA: DAAM1 Antibody (4H3) [H00023002-M03] - Detection limit for recombinant GST tagged DAAM1 is approximately 1ng/ml as a capture antibody.
Proximity Ligation Assay: DAAM1 Antibody (4H3) [H00023002-M03]

Proximity Ligation Assay: DAAM1 Antibody (4H3) [H00023002-M03]

Proximity Ligation Assay: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of protein-protein interactions between RHOA and DAAM1. HeLa cells were stained with anti-RHOA rabbit purified polyclonal 1:1200 and anti-DAAM1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Applications for DAAM1 Antibody (4H3) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA. Use in Immunoprecipitation reported in scientific literature (PMID: 29972794).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DAAM1

Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein. Evidence of alternative splicing has been observed for this gene but the full-length nature of these variants has not been determined.

Alternate Names

disheveled-associated activator of morphogenesis 1, dishevelled associated activator of morphogenesis 1, dishevelled-associated activator of morphogenesis 1, KIAA0666FLJ41657

Entrez Gene IDs

23002 (Human)

Gene Symbol

DAAM1

OMIM

606626 (Human)

UniProt

NP_055807 (Human)

Additional DAAM1 Products

Product Documents for DAAM1 Antibody (4H3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for DAAM1 Antibody (4H3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DAAM1 Antibody (4H3) - Azide and BSA Free

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