DAO Antibody (1G4U2)

Novus Biologicals | Catalog # NBP3-33326

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 1G4U2
View all D-Amino Acid Oxidase Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DAO (NP_001908.3).

Sequence:
MRVVVIGAGVIGLSTALCIHERYHSVLQPLDIKVYADRFTPLTTTDVAAGLWQPYLSDPNNPQEADWSQQTFDYLLSHVHSPNAENLGLFLISGYNLFHE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DAO Antibody (1G4U2)

DAO Antibody (1G4U2)

Western Blot: DAO Antibody (1G4U2) [NBP3-33326] -

Western Blot: DAO Antibody (1G4U2) [NBP3-33326] - Western blot analysis of various lysates using DAO Rabbit mAb at1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
DAO Antibody (1G4U2)

Western Blot: DAO Antibody (1G4U2) [NBP3-33326] -

Western Blot: DAO Antibody (1G4U2) [NBP3-33326] - Western blot analysis of various lysates using DAO Rabbit mAb at1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.

Applications for DAO Antibody (1G4U2)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:2000 - 1:20000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: D-Amino Acid Oxidase

The DAO gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adeninedinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactiveon the naturally occ

Long Name

D-Amino Acid Oxidase

Alternate Names

DAAO, DAMOX, DAO, OXDA

Gene Symbol

DAO

Additional D-Amino Acid Oxidase Products

Product Documents for DAO Antibody (1G4U2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DAO Antibody (1G4U2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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