Home / Mitochondrial Membrane Potential Disruption Assay / DePsipher

DePsipher

Catalog #: 6300-100-01
2 Citations Datasheet / COA / SDS
Reagent for detecting changes in mitochondrial membrane potential

Discontinued Product

6300-100-01 has been discontinued.
View all Mitochondrial Membrane Potential Disruption Assay products.
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Citations (2)
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DePsipher Summary

DePsipher Reagent can be used to evaluate the viability of a cell population, quickly estimate the effect of drugs or other cytotoxins on a cell population, and detect early apoptosis in known models.
 

Key Benefits

• Simple addition of DePsipher Reagent to media or reaction buffer.
• Unique Stabilizer Solution improves results.
• Assay takes only 20 minutes.
• Compatible with epifluorescence, confocal microscopy, or flow cytometry analysis.

How is the DePsipher Reagent Used?

The DePsipher reagent is easy to use. Simply resuspend the reagent in reaction buffer or culture media (with or without the stabilizer solution), add to your cells, incubate for 15 to 20 minutes, wash and analyze by flow cytometry or microscopy. Visualization by microscopy allows a rapid inspection and qualification of apoptosis. Flow cytometric analysis allows easy quantitation of cell death as evidenced by mitochondrial potential breakdown.

Specifications

Shipping Conditions
The product is shipped with dry ice or polar packs.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Species
N/A

Limitations

For research use only. Not for diagnostic use.

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Citations for DePsipher

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. ULK1 inhibition as a targeted therapeutic strategy for FLT3-ITD-mutated acute myeloid leukemia
    Authors: DY Hwang, JI Eom, JE Jang, HK Jeung, H Chung, JS Kim, JW Cheong, YH Min
    J. Exp. Clin. Cancer Res., 2020-05-11;39(1):85.  2020-05-11
  2. Essential role of TAK1 in regulating mantle cell lymphoma survival.
    Authors: Buglio D, Palakurthi S, Byth K, Vega F, Toader D, Saeh J, Neelapu S, Younes A
    Blood, 2012-05-30;120(2):347-55.  2012-05-30

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