DIAPH3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35125

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DIAPH3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 630-849 of human DIAPH3 (NP_112194.2).

Sequence:
KYPDILNFVDDLEPLDKASKVSVETLEKNLRQMGRQLQQLEKELETFPPPEDLHDKFVTKMSRFVISAKEQYETLSKLHENMEKLYQSIIGYYAIDVKKVSVEDFLTDLNNFRTTFMQAIKENIKKREAEEKEKRVRIAKELAERERLERQQKKKRLLEMKTEGDETGVMDNLLEALQSGAAFRDRRKRTPMPKDVRQSLSPMSQRPVLKVCNHGNKPYL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

137 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DIAPH3 Antibody - BSA Free

DIAPH3 Antibody

Western Blot: DIAPH3 Antibody [NBP3-35125] -

Western Blot: DIAPH3 Antibody [NBP3-35125] - Western blot analysis of various lysates using DIAPH3 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Applications for DIAPH3 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DIAPH3

DIAPH3 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to themembrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, andtranscriptional activation of the serum r

Alternate Names

DIAP3, diaphanous homolog 3 (Drosophila), diaphanous-related formin 3, DKFZp434C0931, DKFZp686A13178, homolog) 3, mDia2, protein diaphanous homolog 3

Gene Symbol

DIAPH3

Additional DIAPH3 Products

Product Documents for DIAPH3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DIAPH3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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