Dihydroethidium

Tocris Bioscience | Catalog # 7642

Fluorescent ROS indicator
Tocris Bioscience
Discontinued Product
7642 has been discontinued. View all Fluorescent Cell Indicators and Sensors products.

Key Product Details

Description

Fluorescent ROS indicator

Wavelength

Excitation = 500 nm, Emission = 582 nm
View all Fluorescent Cell Indicators and Sensors »

Product Description

Dihydroethidium is a fluorescent reactive oxygen species indicator. Cell permeable and suitable for use in live cells. Can be used with Mito-HE (Cat. No. 7641) to measure superoxide in living cells. Excitation/emission maxima λ 350/595 nm

Optical Data

Dihydroethidium Dye Spectra
Emission Color Yellow
λabs 500 nm
λem 582 nm
Extinction Coefficient (ε) 14000 M-1cm-1
Cell Permeable Yes
Application Flow Cytometry, Confocal Imaging

Spectra Viewer

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Product Specifications for Dihydroethidium

Molecular Weight

315.41

Formula

C21H21N3

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

5-Ethyl-5,6-dihydro-6-phenyl-3,8-phenanthridinediamine

CAS Number

104821-25-2

PubChem ID

128682

InChI Key

XYJODUBPWNZLML-UHFFFAOYSA-N

SMILES

NC=1C=CC=2C3=CC=C(N)C=C3C(C=4C=CC=CC4)N(C2C1)CC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 3.15 10

Preparing Stock Solutions for Dihydroethidium

The following data is based on the product molecular weight 315.41.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 31.70 mL 158.52 mL 317.05 mL
0.5 mM 6.34 mL 31.70 mL 63.41 mL
1 mM 3.17 mL 15.85 mL 31.70 mL
5 mM 0.63 mL 3.17 mL 6.34 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Dihydroethidium

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Dihydroethidium

For research use only

Citations for Dihydroethidium

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