DNA Ligase I Antibody (1A9)

Novus Biologicals | Catalog # NBP3-07248

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1A9
View all DNA Ligase I Primary Antibodies »

Product Specifications

Immunogen

Full-length native bovine DNA Ligase I protein (Uniprot: P18858)

Localization

Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08267)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for DNA Ligase I Antibody (1A9)

Application
Recommended Usage

Western Blot

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: DNA Ligase I

DNA ligase 1 catalyzes the joining of double-stranded DNA ends during replication, recombination, and repair. Structurally, DNA ligase 1 is toroidal and encircles nicked DNA substrates. The 9-1-1 complex, a DNA damage checkpoint complex, has been reported to associate with DNA ligase 1 and stimulate ligase activity. Mutations in DNA ligase 1 result in immunodeficiency and increased sensitivity to DNA-damaging agents.

Long Name

DNA ligase 1

Alternate Names

EC 6.5.1.1

Gene Symbol

LIG1

UniProt

P18858 (Human)

Additional DNA Ligase I Products

Product Documents for DNA Ligase I Antibody (1A9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DNA Ligase I Antibody (1A9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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